A historical review of the techniques of recovery of parasites for their detection in human stools
Rev. Soc. Bras. Med. Trop
;
53: e20190535, 2020. tab
Article
in English
| LILACS, ColecionaSUS, SES-SP
| ID: biblio-1136801
ABSTRACT
Abstract Since the early 20th century, the detection of intestinal parasites has improved with the development of several techniques for parasitic structures recovery and identification, which differ in sensitivity, specificity, practicality, cost, and infrastructure demand. This study aims to review, in chronological order, the stool examination techniques and discuss their advantages, limitations, and perspectives, and to provide professionals and specialists in this field with data that lays a foundation for critical analysis on the use of such procedures. The concentration procedures that constitute the main techniques applied in routine research and in parasitological kits are a) spontaneous sedimentation; b) centrifugation-sedimentation with formalin-ethyl acetate; and c) flotation with zinc sulfate solution. While selecting a technique, one should consider the purpose of its application and the technical-operational, biological, and physicochemical factors inherent in the procedures used in stool processing, which may restrict its use. These intrinsic limitations may have undergone procedural changes driven by scientific and technological development and by development of alternative methods, which now contribute to the improvement of diagnostic accuracy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Parasitology
/
Specimen Handling
/
Feces
/
Intestinal Diseases, Parasitic
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Year:
2020
Type:
Article
Institution/Affiliation country:
Immunocamp Ciência e Tecnologia/BR
/
Universidade Estadual Paulista/BR
/
Universidade Estadual de Campinas/BR
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