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Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance, mitochondrial dysfunction and oxidative stress
Peng, Yan; Yang, Xinming; Luo, Xi; Liu, Chunhong; Cao, Xia; Wang, Hongyan; Guo, Liyuan.
  • Peng, Yan; Heilongjiang University of Chinese Medicine. The First Affiliated Hospital. Disease Prevention Center. Harbin. CN
  • Yang, Xinming; Heilongjiang University of Chinese Medicine. The First Affiliated Hospital. Department of Obstetrics and Gynecology. Harbin. CN
  • Luo, Xi; Heilongjiang University of Chinese Medicine. The First Affiliated Hospital. Department of Obstetrics and Gynecology. Harbin. CN
  • Liu, Chunhong; Heilongjiang University of Chinese Medicine. College of Basic Medicine Sciences. Harbin. CN
  • Cao, Xia; Heilongjiang University of Chinese Medicine. Document Retrival Center. Harbin. CN
  • Wang, Hongyan; Cancer Hospital of Harbin Medical University. Department of Gynecological Oncology. Harbin. CN
  • Guo, Liyuan; Cancer Hospital of Harbin Medical University. Department of Gynecological Oncology. Harbin. CN
Biol. Res ; 53: 50, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142417
ABSTRACT

BACKGROUND:

Acupuncture, a therapy of traditional Chinese medicine, is confirmed to exert the therapeutic action on polycystic ovary syndrome (PCOS). However, the detailed therapeutic mechanisms of acupuncture in PCOS remain ambiguous. In this study, we further investigated whether electroacupuncture (EA) alleviated PCOS-like symptoms in rats via regulating a metabolic regulator, sterol regulatory element binding protein-1 (SREBP1).

Methods:

The PCOS-like rat model was built by hypodermic injection with dehydroepiandrosterone (DHEA). The rats were subjected to EA intervention (ST29 and SP6 acupuncture points) for 5 weeks. Primary granulosa cells were isolated from control and PCOS-like rats for evaluating insulin resistance, mitochondrial dysfunction and oxidative stress in vitro.

RESULTS:

The expression of SREBP1 was increased in PCOS-like rats, which was suppressed by EA treatment. In addition, lentivirus-mediated overexpression of SREBP1 restrained EA treatment-induced improvement in pathological changes, serum hormone levels and insulin resistance in rats. In addition, overexpression of SREBP1 repressed insulin-stimulated phosphorylation of insulin receptor ß (IR) and AKT in primary granulosa cells. Moreover, upregulation of SREBP1 further exacerbated mitochondrial dysfunction and oxidative stress in granulosa cells isolated from PCOS-like rats. Mechanically, EA treatment suppressed SREBP1 expression through inducing the activation of AMP-activated protein kinase (AMPK) signaling pathway in PCOS-like rats.

CONCLUSION:

EA intervention alleviated PCOS-like symptoms in rats via improving IR, mitochondrial dysfunction and oxidative stress through regulating SREBP1, a lipid metabolism regulator. Our findings illuminate the novel protective mechanisms of EA in the treatment of PCOS.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Polycystic Ovary Syndrome / Insulin Resistance / Electroacupuncture / Oxidative Stress / Sterol Regulatory Element Binding Protein 1 / Mitochondria Type of study: Prognostic study Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Cancer Hospital of Harbin Medical University/CN / Heilongjiang University of Chinese Medicine/CN

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Full text: Available Index: LILACS (Americas) Main subject: Polycystic Ovary Syndrome / Insulin Resistance / Electroacupuncture / Oxidative Stress / Sterol Regulatory Element Binding Protein 1 / Mitochondria Type of study: Prognostic study Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Cancer Hospital of Harbin Medical University/CN / Heilongjiang University of Chinese Medicine/CN