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Effect of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2) transfection of rBMSCs compounded with platelet-rich plasma on adipogenic differentiation
Sun, Jin; Jiang, Xin; Luo, Junnan; Zhao, Liheng; Xu, Zuhua; Xiao, Wende.
  • Sun, Jin; Huizhou Third Peoples Hospital, Guangzhou Medical University. Department of Orthopedics. Huizhou. CN
  • Jiang, Xin; Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology. Department of Orthopedics. Guangzhou. CN
  • Luo, Junnan; Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology. Department of Orthopedics. Guangzhou. CN
  • Zhao, Liheng; Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology. Department of Orthopedics. Guangzhou. CN
  • Xu, Zuhua; Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology. Department of Orthopedics. Guangzhou. CN
  • Xiao, Wende; Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology. Department of Orthopedics. Guangzhou. CN
Braz. j. med. biol. res ; 54(2): e9944, 2021. tab, graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1142581
Responsible library: BR1.1
ABSTRACT
The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat bone marrow mesenchymal stem cells (rBMSCs) and then compounded with platelet-rich plasma (PRP). To achieve rBMSCs, the osteoporosis model of rats was established, and then the rBMSCs from the rats were isolated and identified. Co-transfection of rBMSCs with PDGF-BB-GFP and BMP-2 and detection of PDGF-BB/BMP-2 expression in transfected BMSCs was assessed by qRT-PCR and western blot, respectively. Moreover, the effect of the two growth factors transfection of rBMSCs on adipogenic differentiation was evaluated by oil red O staining and western blot, respectively. Finally, construction of the two growth factors transfection of rBMSCs compounded with PRP and detection of adipogenic differentiation were assessed by oil red O staining, CCK-8, and western blot, respectively. In vitro studies revealed that the two growth factors transfection of rBMSCs compounded with PRP promoted cell viability and inhibited adipogenic differentiation and could be promising for inhibiting adipogenic differentiation.
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Full text: Available Index: LILACS (Americas) Main subject: Cell Differentiation / Adipose Tissue / Platelet-Rich Plasma / Bone Morphogenetic Protein 2 / Mesenchymal Stem Cells / Becaplermin Limits: Animals Language: English Journal: Braz. j. med. biol. res Year: 2021 Type: Article Institution/Affiliation country: Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology/CN / Huizhou Third Peoples Hospital, Guangzhou Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Cell Differentiation / Adipose Tissue / Platelet-Rich Plasma / Bone Morphogenetic Protein 2 / Mesenchymal Stem Cells / Becaplermin Limits: Animals Language: English Journal: Braz. j. med. biol. res Year: 2021 Type: Article Institution/Affiliation country: Guangzhou First Peoples Hospital, School of Medicine, South China University of Technology/CN / Huizhou Third Peoples Hospital, Guangzhou Medical University/CN