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Morphometric and molecular muscle remodeling after passive stretching in elderly female rats
Martins, Hilana Rickli Fiuza; Zotz, Talita G. Gnoato; Messa, Sabrina Peviani; Capriglione, Luiz Guilherme A; Zotz, Rafael; Noronha, Lucia; Azevedo, Marina Louise Viola De; Gomes, Anna Raquel Silveira.
  • Martins, Hilana Rickli Fiuza; Universidade Estadual do Centro-Oeste. Universidade Federal do Paraná. Guarapuava. BR
  • Zotz, Talita G. Gnoato; Universidade Federal do Paraná. Departamento de Prevencao e Reabilitacao em Fisitoterapia. BR
  • Messa, Sabrina Peviani; Faculdade de Ciencias Biomedicas de Cacoal. BR
  • Capriglione, Luiz Guilherme A; Universidade do Paraná. Programa de Ciencias da Saude. Curitiba. BR
  • Zotz, Rafael; Pontificia Universidade Catolica do Paraná. Bacharel em zootecnia. Curitiba. BR
  • Noronha, Lucia; Pontificia Universidade Católica do Paraná. Programa de Pos-Graduacao em Ciencias da Saude. Laboratorio da Escola de Medicina e Patologia Experimental. BR
  • Azevedo, Marina Louise Viola De; Pontificia Universidade Catolica do Paraná. Departamento de Patologia Experimental. Curitiba. BR
  • Gomes, Anna Raquel Silveira; Universidade Federal do Paraná. Programa de Pos-Graduacao em Educacao Fisica. Departamento de Prevencao e Reabilitacao em Fisitoterapia. BR
Clinics ; 75: e1769, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142762
ABSTRACT

OBJECTIVES:

To determine the effects of three sessions of a passive stretching exercise protocol on the muscles of elderly female rats.

METHODS:

The effects of the stretching exercises on the soleus muscle were analyzed using immunohistochemistry [tissue inhibitors of matrix metalloproteinases (TIMP), the tumor necrosis factor-alpha (TNF-α), and the gene expression levels using real-time PCR of the transforming growth factor-beta 1 (TGF-β1), collagen type 1 (COL1), and collagen type 3 (COL3)]. Fifteen 26-month-old female Wistar rats were randomly divided into two groups, namely, Stretching (SG, n=8) and Control (CG, n=7). The passive mechanical stretching protocol consisted of a set of 4 1-minute repetitions, with 30 seconds between each repetition (total treatment of 4 minutes), three times a week for 1 week.

RESULTS:

Immunohistochemical analysis revealed an increase of 71.4% in the TNF-α (p=0.04) gene expression levels for the SG and a 58% decrease in the TGF-β1 gene expression levels (p=0.005) in the SG compared to that in the CG. No significant differences were observed between the groups for the immunostaining of TIMP-1 or the gene expression levels of COL1 and COL3.

CONCLUSION:

Three sessions of static stretching reduced the gene expression level of TGF-β1, which, owing to its anti-fibrotic role, might contribute to the remodeling of the intramuscular connective tissue of the aging muscle. In addition, immunostaining revealed that TNF-α levels increased in the aging muscle tissue in response to stretching, indicating its effect on stimulating extracellular matrix degradation. These outcomes have important clinical implications in reinforcing the use of stretching exercises in the elderly, considering that the aging muscle presents an infiltration of connective tissue.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Muscle Stretching Exercises Type of study: Practice guideline Limits: Animals / Female / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Ciencias Biomedicas de Cacoal/BR / Pontificia Universidade Catolica do Paraná/BR / Pontificia Universidade Católica do Paraná/BR / Universidade Estadual do Centro-Oeste/BR / Universidade Federal do Paraná/BR / Universidade do Paraná/BR

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Full text: Available Index: LILACS (Americas) Main subject: Muscle Stretching Exercises Type of study: Practice guideline Limits: Animals / Female / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Ciencias Biomedicas de Cacoal/BR / Pontificia Universidade Catolica do Paraná/BR / Pontificia Universidade Católica do Paraná/BR / Universidade Estadual do Centro-Oeste/BR / Universidade Federal do Paraná/BR / Universidade do Paraná/BR