Serologic strategy in detecting RHD altered alleles in Brazilian blood donors
Hematol., Transfus. Cell Ther. (Impr.)
;
42(4): 365-372, Oct.-Dec. 2020. tab, graf, ilus
Article
in English
| LILACS
| ID: biblio-1142967
ABSTRACT
ABSTRACT Background:
We evaluated different technological approaches and anti-D clones to propose the most appropriate serologic strategy in detecting the largest numbers of D variants in blood donors.Methods:
We selected 101 samples from Brazilian blood donors with different expressions of D in our donor routine. The tests were performed in immediate spin (IS) with eleven commercially available anti-D reagents in a tube and microplate. The D confirmatory tests for the presence of weak D included the indirect antiglobulin test (IAT) in a tube, gel and solid-phase red blood cell adherence (SPRCA). All DNA samples were extracted from peripheral blood and the D variants were classified using different molecular assays.Results:
The RHD variants identified by molecular analysis included weak D types (1, 2, 3, 11 and 38) and partial Ds (DAR1.2, DAR1, DAR3.1, DAU0, DAU2, DAU4, DAU5, DAU6, DMH and DVII). The monoclonal-monoclonal blend RUM-1/MS26 was the best anti-D reagent used in detecting the D antigen in the IS phase in a tube, reacting with 83.2% of the D variants, while the anti-D blend D175 + 415 was the best monoclonal antibody (MoAb) used in a microplate to minimize the need for an IAT, reacting with 83.2% of the D variants. The D confirmatory tests using SPRCA showed a reactivity (3 - 4+) with 100% of the D variant samples tested.Conclusion:
Our results show that, even using sensitive methods and MoAbs to ensure the accurate assignment of the D antigen, at least 17% of our donor samples need a confirmatory D test in order to avoid alloimmunization in D-negative patients.
Full text:
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Index:
LILACS (Americas)
Main subject:
Rh-Hr Blood-Group System
/
Blood Donors
/
Serotyping
/
Alleles
/
Hemagglutination
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Hematol., Transfus. Cell Ther. (Impr.)
Journal subject:
Hematologia
/
TransfusÆo de Sangue
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Associação Beneficente de Coleta de Sangue Colsan/BR
/
Hemocentro de São José do Rio Preto/BR
/
Universidade Estadual de Campinas - UNICAMP/BR
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