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Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil
Gonçalves, Lucas Pantoja; Santos, Thiago Vasconcelos dos; Campos, Marliane Batista; Lima, Luciana Vieira do Rêgo; Ishikawa, Edna Aoba Yassui; Silveira, Fernando Tobias; Ramos, Patrícia Karla Santos.
  • Gonçalves, Lucas Pantoja; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
  • Santos, Thiago Vasconcelos dos; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
  • Campos, Marliane Batista; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
  • Lima, Luciana Vieira do Rêgo; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
  • Ishikawa, Edna Aoba Yassui; Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém. BR
  • Silveira, Fernando Tobias; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
  • Ramos, Patrícia Karla Santos; Instituto Evandro Chagas. Laboratório de Leishmanioses Prof. Dr. Ralph Lainson. Seção de Parasitologia. Ananindeua. BR
Rev. Soc. Bras. Med. Trop ; 53: e20200255, 2020. tab, graf
Article in English | SES-SP, ColecionaSUS, LILACS | ID: biblio-1143862
ABSTRACT
Abstract

INTRODUCTION:

In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region.

METHODS:

Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases.

RESULTS:

ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%).

CONCLUSIONS:

To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
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Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Leishmaniasis, Cutaneous / Leishmania Type of study: Prognostic study / Screening study Limits: Humans Country/Region as subject: North America / South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Year: 2020 Type: Article Institution/Affiliation country: Instituto Evandro Chagas/BR / Universidade Federal do Pará/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Leishmaniasis, Cutaneous / Leishmania Type of study: Prognostic study / Screening study Limits: Humans Country/Region as subject: North America / South America / Brazil Language: English Journal: Rev. Soc. Bras. Med. Trop Year: 2020 Type: Article Institution/Affiliation country: Instituto Evandro Chagas/BR / Universidade Federal do Pará/BR