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Filter paper performance in PCR for cutaneous leishmaniasis diagnosis
Mota, Camila Alves; Venazzi, Eneide Aparecida Sabaini; Zanzarini, Paulo Donizeti; Aristides, Sandra Mara Alessi; Lonardoni, Maria Valdrinez Campana; Silveira, Thaís Gomes Verzignassi.
  • Mota, Camila Alves; Universidade Estadual de Maringá. Programa de Pós-Graduação em Biociências e Fisiopatologia. Maringá. BR
  • Venazzi, Eneide Aparecida Sabaini; Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina. Laboratório de Parasitologia Clínica. Maringá. BR
  • Zanzarini, Paulo Donizeti; Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina. Laboratório de Leishmanioses. Maringá. BR
  • Aristides, Sandra Mara Alessi; Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina. Laboratório de Leishmanioses. Maringá. BR
  • Lonardoni, Maria Valdrinez Campana; Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina. Laboratório de Leishmanioses. Maringá. BR
  • Silveira, Thaís Gomes Verzignassi; Universidade Estadual de Maringá. Departamento de Análises Clínicas e Biomedicina. Laboratório de Leishmanioses. Maringá. BR
Rev. Soc. Bras. Med. Trop ; 54: e00472020, 2021. tab, graf
Article in English | SES-SP, ColecionaSUS, LILACS | ID: biblio-1143886
ABSTRACT
Abstract

INTRODUCTION:

The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis.

METHODS:

Lesion scrapings from 48 patients were collected and analyzed for PCR.

RESULTS:

PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%.

CONCLUSIONS:

FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.
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Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Leishmaniasis, Cutaneous Type of study: Diagnostic study / Practice guideline Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Year: 2021 Type: Article Institution/Affiliation country: Universidade Estadual de Maringá/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leishmania braziliensis / Leishmaniasis, Cutaneous Type of study: Diagnostic study / Practice guideline Limits: Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Year: 2021 Type: Article Institution/Affiliation country: Universidade Estadual de Maringá/BR