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Cytotoxic Effects of Bulk-Fill Composites on L929 Fibroblast Cells / Efeitos citotóxicos dos compósitos bulk-fill em células de fibroblastos L929
Aydin, Numan; Karaoglanoglu, Serpil; Oktay, Elif Aybala; Süloglu, Aysun Kiliç.
  • Aydin, Numan; University of Health Sciences. Gulhane Faculty of Dentistry. Department of Restorative Dental Treatment. Ankara. TR
  • Karaoglanoglu, Serpil; University of Health Sciences. Gulhane Faculty of Dentistry. Department of Restorative Dental Treatment. Ankara. TR
  • Oktay, Elif Aybala; University of Health Sciences. Gulhane Faculty of Dentistry. Department of Restorative Dental Treatment. Ankara. TR
  • Süloglu, Aysun Kiliç; Hacettepe University. Faculty of Science. Department of Biology. Ankara. TR
Braz. dent. sci ; 24(1): 1-9, 2021. tab, ilus
Article in English | BBO, LILACS | ID: biblio-1145441
ABSTRACT

Objective:

Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and

Methods:

In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n3) of which surface area was calculated according to ISO 10993-12 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 11 and 12 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05).

Results:

When the 11 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 11 and 12 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test.

Conclusions:

Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts. (AU)
RESUMO

Objetivo:

Ao contrário das resinas compostas tradicionais, as resinas compostas bulk-fill podem ser polimerizadas como camadas mais espessas. Este estudo visa investigar in vitro os efeitos citotóxicos de várias resinas compostas bulk-fill em células de fibroblastos de camundongo L929.Material e

Métodos:

Em nosso estudo, seis resinas tipo bulk fill e uma resina composta convencional foram usadas. Amostras de resina composta (8 × 4 mm) foram preparadas em gabinete estéril usando um molde de vidro e polimerizado com um dispositivo de luz LED (DTE LUX E, Alemanha). Amostras compostas (n=3) cuja área de superfície foi calculada de acordo com os padrões ISO 10993-122012 (3cm2/ml), foram mantidas em meio e incubadas por 24 h e 72 h a 37 ºC, seus extratos foram filtrados na Proporção de 11 e 12 e foram acondicionados em cultura de células de fibroblastos de camundongo L929. A viabilidade celular foi examinada pelo ensaio MTT e a morte celular pelo teste LDH. Os resultados de viabilidade celular foram avaliados usando o teste de análise de variância (ANOVA) um fator (p <0,05).

Resultados:

Quando os extratos foram plaqueados na proporção 11 de amostras de compósito bulk-fill de 4 mm de espessura com as células de fibroblastos de camundongo L929, as taxas de viabilidade celular mostraram diferenças significativas em comparação com o grupo controle no final de 24 h e 72 h (exceto para Estelite Bulk Fluxo de enchimento). Embora os extratos das amostras compostas testadas na proporção de 11 e 12 ao final de 72 horas tenham causado uma diminuição na viabilidade das células de fibroblastos de camundongo L929, a taxa de viabilidade celular apenas do compósito de preenchimento total contendo PRG e o compósito convencional permaneceram abaixo a taxa de viabilidade celular (70%) especificada nas normas ISO. Os compósitos de preenchimento a granel não produziram efeitos tóxicos (exceto Beautifil Bulk Restorative) de acordo com o teste de LDH.

Conclusão:

Apesar de diminuir em geral a viabilidade celular, as resinas compostas bulk-fill usadas em camadas de 4 mm de espessura forneceram taxas de viabilidade celular acima do nível aceitável, exceto o compósito bulk fill contendo PRG (Beautifil Bulk Restorative), que foi citotóxico para fibroblastos de camundongos L929 (AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Composite Resins / Toxicity / Fibroblasts Limits: Animals Language: English Journal: Braz. dent. sci Journal subject: Dentistry / Sa£de Bucal Year: 2021 Type: Article Affiliation country: Turkey Institution/Affiliation country: Hacettepe University/TR / University of Health Sciences/TR

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Full text: Available Index: LILACS (Americas) Main subject: Composite Resins / Toxicity / Fibroblasts Limits: Animals Language: English Journal: Braz. dent. sci Journal subject: Dentistry / Sa£de Bucal Year: 2021 Type: Article Affiliation country: Turkey Institution/Affiliation country: Hacettepe University/TR / University of Health Sciences/TR