Development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in capsule formulation
Rev. ciênc. farm. básica apl
;
42: 8, 20210101.
Article
in English
|
LILACS-Express
| LILACS
| ID: biblio-1148221
ABSTRACT
Objective. The aim of this study was the development and validation of an UV-Vis spectrophotometric method for the quantification of oclacitinib in commercial capsule formulation since pharmacopeias have not yet provided an official monograph for this drug. Methods. The parameters linearity, limit of detection, limit of quantitation, specificity, precision, accuracy, and robustness were determined according to Brazilian and international guidelines. Results. Linearity was determined for the analytical range of 5-15 µg/mL, and a limit of detection of 1.18 µg/mL and limit of quantification of 3.58 µg/mL were obtained. The method was selective and the precision was demonstrated through repeatability and intermediate precision, with relative standard deviations of 1.96% and 1.78%, respectively. In its turn, accuracy presented recovery percentages of 98.32-100.91%. All robustness and sample stability (48 h at 25 °C) results revealed no statistical variation among the groups. Conclusions. The presented method is suitable for the quantification of oclacitinib in commercial capsule formulation.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. ciênc. farm. básica apl
Journal subject:
Pharmacology
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Departamento de Farmácia/BR
/
Programa de Pós-graduação em Ciências Farmacêuticas/BR
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