Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris
Vaccimonitor (La Habana, Print)
;
30(1)ene.-abr. 2021. tab, graf
Article
in English
| LILACS, CUMED
| ID: biblio-1150249
ABSTRACT
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies)AU)
RESUMEN
El objetivo de este trabajo es la expresión de la región PreS2-S del antígeno de superficie del virus de la hepatitis B en la levadura Pichia pastoris. Se clonó un fragmento de ADNc que codifica la proteína PreS2-S del VHB en vectores de transferencia de levadura. A partir de los nuevos plásmidos clonados pPIC3.5-PreS2-S (8707 pb) y pPIC9-PreS2-S (8980 pb) se obtuvieron las cepas recombinantes de P. pastoris productoras de la región PreS2-S del antígeno de superficie del VHB. La electroforesis PAGE y la inmunotransferencia de la proteína PreS2-S recombinante obtenida confirman el peso molecular (34 kDa) y la alta especificidad a los anticuerpos contra el VHB(AU)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Recombinant Proteins
/
Hepatitis B virus
/
Vaccines, DNA
Limits:
Humans
Language:
English
Journal:
Vaccimonitor (La Habana, Print)
Journal subject:
Molecular Biology
/
Epidemiology
/
Microbiology
/
Vacinas
Year:
2021
Type:
Article
Affiliation country:
Uzbekistan
Institution/Affiliation country:
Acad. S. Yu. Yunusov Institute of the Chemistry of Plant Substances/UZ
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