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In vitro efficacy of five pediatric toothpastes in the inhibition of streptococcus mutans ATCC 25175 / Eficacia in vitro de cinco pastas dentales pediátricas en la inhibición de streptococcus mutans ATCC 25175
Miñano, José; Espinoza, Maria V.
  • Miñano, José; Universidad Privada Antenor Orrego. Trujillo. PE
  • Espinoza, Maria V; Universidad Privada Antenor Orrego. Trujillo. PE
J. oral res. (Impresa) ; 9(1): 29-35, feb. 28, 2020. ilus, tab
Article in English | LILACS | ID: biblio-1151424
ABSTRACT
The present in vitro study compared the inhibitory action of five pediatric toothpastes against Streptococcus mutans ATCC 25175. Materials and

Methods:

Cross-sectional, comparative and experimental study. The microorganism Streptococcus mutans ATCC 25175 was inoculated onto solid culture medium of Müeller-Hinton supplemented with blood, then the plates were inoculated with five pediatric toothpastes Oral B Stages, Colgate Smiles, Aquafresh My Little Teeth, Dentito and Denture Kids. Samples were incubated at 37°C for 48 hours. Subsequently the inhibition halos were measured; the experiment was repeated 20 times for each sample. Statistical analysis was performed with ANOVA complemented with Tukey's test.

Results:

Oral B Stages yielded a mean inhibitory halo of 23.2mm, Colgate Smiles an average of 21.7mm, Aquafresh My Little Teeth of 13.6mm, Dentito of 18.5mm, and Denture Kids a mean of 23.0mm. When performing the ANOVA test, it was found that there was a significant difference in the inhibitory action of pediatric toothpastes (p<0.005) and when using Tukey's multiple comparison test, Oral B and Denture Kids presented similar inhibitory action.

Conclusion:

All toothpastes presented inhibitory action against Streptococcus mutans ATCC 25175. A significant difference between their effectiveness was observed. Oral B Stages showed the greatest degree of inhibition.
RESUMEN
El presente estudio in vitrocomparo la eficacia inhibitoria de cinco pastas dentales pediátricas frente a la bacteria Streptococcus mutansATCC25175. Material y

Métodos:

El estudio fue transversal, comparativo y experimental. Se inoculó Streptococcus mutansATCC 25175 en un medio de cultivo Müeller-Hinton complementado con sangre, luego a las placas cultivadas se le inocularon cincopastas dentales pediátricas Oral B Stages, Colgate Smiles, Aquafresh My LittleTeeth, Dentito y Denture Kids. Se incubó a 37ºC por 48 horas y posteriormente semidió los halos de inhibición, se replicó el experimento 20 veces para cada uno. El análisis estadístico se realizó con el test de ANVA complementado con el test de Tukey.

Resultado:

Oral B Stages generó una media inhibitoria de 23,2mm, ColgateSmiles una media de 21,7mm, Aquafresh My Little Teeh de 13,6mm, Dentitode 18,5mm y finalmente Denture Kids una media de 23,0mm. Al realizar laprueba ANOVA se encontró que hay diferencia significativa en la acción inhibitoriade las pastas dentales pediátricas (p<0.005) y al emplear la prueba Tukey (comparación de múltiples) la pasta Oral B y Denture Kids presentaron acción inhibidora similar.

Conclusión:

Todas las pastas presentaron acción inhibitoria sobre Streptococcus mutansATCC 25175 existiendo diferencia significativa entre la efectividad de estas, con la pasta Oral B Stages demuestrando mayor acción inhibitoria.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Streptococcus mutans / Toothpastes Type of study: Observational study / Prevalence study / Risk factors Limits: Child / Humans Country/Region as subject: South America / Peru Language: English Journal: J. oral res. (Impresa) Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Peru Institution/Affiliation country: Universidad Privada Antenor Orrego/PE

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Full text: Available Index: LILACS (Americas) Main subject: Streptococcus mutans / Toothpastes Type of study: Observational study / Prevalence study / Risk factors Limits: Child / Humans Country/Region as subject: South America / Peru Language: English Journal: J. oral res. (Impresa) Journal subject: Dentistry Year: 2020 Type: Article Affiliation country: Peru Institution/Affiliation country: Universidad Privada Antenor Orrego/PE