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Garlic oil as a fight against histological and oxidative stress abnormalities in Wistar rats after oral inoculation of Anisakis spp. Type II (L3) (Nematoda) / [O uso do óleo de alho na luta contra anormalidades histológicas e de estresse oxidativo em ratos Winstar após inoculação oral de Anisakis spp. Tipo II (L3) (Nematoda)]
Morsy, K; Sheraba, N; Alhamhoom, Y; Dajem, S. B; Al-Kahtani, M; Shati, A; Alfaifi, M; Elbehairi, S. E; El-Mekkawy, H; El-Kott, A; Ezzat, A; Meferij, M. B; Saber, S.
  • Morsy, K; King Khalid University. Faculty of Science. Abha. SA
  • Sheraba, N; King Khalid University. College of Pharmacy. Abha. SA
  • Alhamhoom, Y; King Khalid University. College of Pharmacy. Abha. SA
  • Dajem, S. B; King Khalid University. Faculty of Science. Abha. SA
  • Al-Kahtani, M; King Khalid University. Faculty of Science. Abha. SA
  • Shati, A; King Khalid University. Faculty of Science. Abha. SA
  • Alfaifi, M; King Khalid University. Faculty of Science. Abha. SA
  • Elbehairi, S. E; King Khalid University. Faculty of Science. Abha. SA
  • El-Mekkawy, H; King Khalid University. Faculty of Science. Abha. SA
  • El-Kott, A; King Khalid University. Faculty of Science. Abha. SA
  • Ezzat, A; King Khalid University. Faculty of Science. Abha. SA
  • Meferij, M. B; Princess Nourah bint Abdelrahman University. College of Science. Riyadh. SA
  • Saber, S; National Organization for Drug Control and Research. Giza. EG
Arq. bras. med. vet. zootec. (Online) ; 73(1): 141-154, Jan.-Feb. 2021. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1153057
ABSTRACT
The consumption of inadequately thermally treated fish is a public health risk due to the possible propagation of Anisakis larvae and their antigenic proteins, the causative agent of the zoonotic disease anisakidosis. The present study demonstrated the physiological and histopathological changes that accompanied an oral inoculation of crude extracts from fresh and thermally treated Anisakis Type II (L3) in Wistar albino rats. Nematode worms were isolated from the marine fish Dicentrarchus labrax. They were examined and taxonomically identified using light and scanning electron microscopy. The study was performed in 6 rat groups a control group (I), a garlic oil (GO) inoculated group (II), a fresh L3 inoculated group (III), a thermally treated L3 inoculated group (IV), a fresh L3 + GO inoculated group (V), and a thermally treated L3 + GO inoculated group (VI). It was observed that rats inoculated with fresh and thermally treated L3 crude extracts showed abnormal oxidative stress markers associated with the destruction of normal architecture of spleen and thymus. GO produced a protective effect in rat groups inoculated with L3 extracts + GO administration via the amelioration of oxidative stress markers, which was confirmed by the marked normal structure of the organs' histology. Cooking of L3 infected fish induced severe physiological and histopathological alterations compared to uncooked infected fish. The administration of garlic before and after fish eating is recommended to avoid the dangerous effect of anisakids, even if they are cooked.(AU)
RESUMO
O consumo de peixes tratados termicamente de forma inadequada é um risco à saúde pública devido à possível propagação das larvas de Anisakis e suas proteínas antigênicas, o agente causador da doença zoonótica anisakidose. O presente estudo demonstrou as alterações fisiológicas e histopatológicas que acompanharam a inoculação oral de extratos brutos de Anisakis Tipo II (L3) frescos e termicamente tratados em ratos Wistar albinos. Vermes nematoides foram isolados do peixe marinho Dicentrarchus labrax e foram examinados e identificados taxonomicamente usando microscopia óptica e eletrônica de varredura. O estudo foi realizado em 6 grupos de ratos grupo controle (I), grupo inoculado com óleo de alho (GO) (II), grupo inoculado com L3 fresco (III), grupo inoculado com L3 tratado termicamente (IV), grupo inoculado com L3 + GO fresco (V), e grupo inoculado com L3 + GO tratado termicamente (VI). Observou-se que ratos inoculados com extrato bruto L3 fresco e tratado termicamente mostraram marcadores de estresse oxidativo anormais associados à destruição da estrutura normal do baço e do timo. GO produziu um efeito protetor em grupos de ratos inoculados com extrato L3 + administração de GO através da melhoria dos marcadores de estresse oxidativo, que foi confirmada pela marcante estrutura normal da histologia dos órgãos. O cozimento de peixes infectados com L3 induziu alterações fisiológicas e histopatológicas graves quando comparado com peixes infectados não cozidos. Recomenda-se a administração de alho antes e depois da ingestão do peixe para evitar o efeito perigoso dos anisakídeos, mesmo se cozidos.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Anisakis / Anisakiasis / Fishes / Garlic Limits: Animals Language: English Journal: Arq. bras. med. vet. zootec. (Online) Year: 2021 Type: Article Institution/Affiliation country: King Khalid University/SA / National Organization for Drug Control and Research/EG / Princess Nourah bint Abdelrahman University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Anisakis / Anisakiasis / Fishes / Garlic Limits: Animals Language: English Journal: Arq. bras. med. vet. zootec. (Online) Year: 2021 Type: Article Institution/Affiliation country: King Khalid University/SA / National Organization for Drug Control and Research/EG / Princess Nourah bint Abdelrahman University/SA