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MicroRNA-369 attenuates hypoxia-induced cardiomyocyte apoptosis and inflammation via targeting TRPV3
Wang, Jinghao; Chen, Xu; Huang, Wei.
  • Wang, Jinghao; the First Affiliated Hospital, Jinan University. Department of Pharmacy. Guangzhou. CN
  • Chen, Xu; Daqing Oilfield General Hospital. Department of Pharmacy. Daqing. CN
  • Huang, Wei; Harbin Medical University-Daqing. Department of Pharmacology. Daqing. CN
Braz. j. med. biol. res ; 54(3): e10550, 2021. graf
Article in English | LILACS | ID: biblio-1153516
ABSTRACT
Hypoxia-induced apoptosis and inflammation play an important role in cardiovascular diseases including myocardial infarction (MI). miR-369 has been suggested to be a key regulator of cardiac fibrosis. However, the role of miR-369 in regulating hypoxia-induced heart injury remains unknown. Our data indicated that miR-369 expression was significantly down-regulated and TRPV3 was significantly up-regulated in myocardial tissue after MI in rats and in hypoxic-treated neonatal rat cardiomyocytes (NRCMs). In addition, we observed that hypoxia significantly promoted apoptosis and the inflammatory response, accompanied by increased caspase-3 activity and the secretion of the cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α. miR-369 overexpression significantly suppressed cell apoptosis and inflammatory factor production triggered by hypoxia, whereas miR-369 inhibition had an opposite effect. Importantly, we identified TRPV3 as a direct target of miR-369-3p. TRPV3 inhibition with small interfering RNA (siRNA) significantly inhibited hypoxia-induced inflammation and apoptosis, which can reverse the injury effects of miR-369 inhibitors. Our findings indicated that miR-369 reduced hypoxia-induced apoptosis and inflammation by targeting TRPV3.
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Full text: Available Index: LILACS (Americas) Main subject: Myocytes, Cardiac Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Daqing Oilfield General Hospital/CN / Harbin Medical University-Daqing/CN / the First Affiliated Hospital, Jinan University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Myocytes, Cardiac Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Daqing Oilfield General Hospital/CN / Harbin Medical University-Daqing/CN / the First Affiliated Hospital, Jinan University/CN