Acellular dermal matrix in skin wound healing in rabbits - histological and histomorphometric analyses
Clinics
;
76: e2066, 2021. graf
Article
in English
| LILACS
| ID: biblio-1153935
ABSTRACT
OBJECTIVES:
To analyze the histology and histomorphometry of healing associated with acellular dermal matrix in skin wounds in rabbits.METHODS:
Twelve male rabbits were divided into two groups the control group (CG) and the matrix group (MG). Three skin wounds with a total area of 20 × 20 mm were created on the dorsal region of each animal. Photographic records of the lesions taken over a 21-day period and use of the ImageJ program allowed calculation of the wound contraction rate. The lesions were biopsied on days 3, 14 and 21 for histomorphometric analysis to define the thicknesses of the dermis and epidermis (hematoxylin-eosin) and calculate the densities of type I and type III collagen (picrosirius).RESULTS:
No significant difference in the healing rate was found between the groups (p>0.05). The MG presented greater epidermal thickness on day 3 (p<0.05) and on days 14 and 21 (p<0.001). The MG presented greater dermal thickness throughout the study period (p<0.05). The type I collagen density was higher in the MG throughout the study period (p<0.05), and the type III collagen density was higher in the MG on days 3 and 14 (p<0.05) and on day 21 (p<0.001).CONCLUSION:
The use of acellular dermal matrix increased the thickness of the dermal and epidermal layers and the amount of type I and III collagen during skin wound healing and did not alter the rate of wound contraction.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Acellular Dermis
Limits:
Animals
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS