Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine
Pesqui. bras. odontopediatria clín. integr
;
21: e0034, 2021. graf
Article
in English
| LILACS, BBO
| ID: biblio-1155006
ABSTRACT
ABSTRACT Objective:
To evaluate the regeneration of mandibular cartilage defect after implantation of human umbilical cord mesenchymal stem cells (hUCMSC) over platelet rich fibrin (PRF) as scaffold. Material andMethods:
20 male Wistar rats were randomly divided into four experimental groups consisting of a control group featuring untreated mandibular defects (C), experimental groups whose mandibular defects were implanted with hUCMSC (E1), mandibular defects implanted with PRF (E2), mandibular defects implanted with hUCMSC and PRF scaffold (E3). The subjects were sacrificed after six weeks of observation for immunohistochemical examination in order to evaluate the expression of Ki67, Sox9, FGF 18, type 2 collagen, and aggrecan, in addition to histology examination to evaluate chondrocyte number and cartilage thickness. Data was analyzed with univariate analysis (ANOVA).Results:
The implantation of hUCMSC and PRF scaffold proved capable of regenerating mandibular cartilage defect through the expression of FGF 18, Sox9, Ki67, chondrosis counts, type 2 collagen, aggrecan, and cartilage thickness. The regeneration were significantly higher in group E3.Conclusion:
Human umbilical cord mesenchymal stem cells in platelet rich fibrin scaffold proved capable of regenerating mandibular cartilage defect.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cartilage
/
Cord Blood Stem Cell Transplantation
/
Regenerative Medicine
/
Mesenchymal Stem Cells
/
Platelet-Rich Fibrin
Type of study:
Evaluation studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Indonesia
Institution/Affiliation country:
Universitas Airlangga/ID
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