Quantification of fecal neutrophils by MPO determination (myeloperoxidase) in patients with invasive diarrhea. Cuantificación de neutrofilos fecales mediante la determinación de MPO (Mieloperoxidasa) en pacientes con diarrea invasiva
Acta gastroenterol. latinoam
; 30(2): 85-7, 2000.
Article
in Es
|
LILACS-Express
| LILACS, BINACIS
| ID: biblio-1157284
Responsible library:
AR40.1
Localization: [{"text": "AR40.1"}]
ABSTRACT
Myeloperoxidase (MPO), a specific polymorphonuclear leukocyte enzyme, has been used previously to quantify the number of neutrophils in tissue. MPO activity was found to be linearly related to the number of neutrophil cells. In an attempt to use this method in leukocytes measuring in stool, fecal MPO was solubilized with hexadecyltrimethylammonium bromide and the MPO activity was measured by a dianisidine-H2O2 assay. Stools from 10 normal subjects and 39 patients with diarrhea produced by enteropathogenic bacteria were examined for leukocytes by MPO activity as well as microscopically using methylene blue stain, MPO activity was positive in 36 patients (92
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Index:
LILACS
Language:
Es
Journal:
Acta gastroenterol. latinoam
Journal subject:
GASTROENTEROLOGIA
Year:
2000
Type:
Article