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Evaluation of red blood cell aggregation in diabetes by computerized image analysis
Foresto, P; D’Arrigo, M; Carreras, L; Cuezzo, R E; Valverde, J; Rasia, R.
  • Foresto, P; s.af
  • D’Arrigo, M; s.af
  • Carreras, L; s.af
  • Cuezzo, R E; s.af
  • Valverde, J; s.af
  • Rasia, R; Laboratorio de Inmuno-Hemorreología, Universidad Nacional de Rosario, Argentina. pforesto@fbioyf.unr.edu.ar.
Medicina (B.Aires) ; 60(5 Pt 1): 570-2, 2000.
Article in Spanish | LILACS, BINACIS | ID: biblio-1165080
ABSTRACT
Red blood cell (RBC) aggregation has been widely studied and its importance is well established in the rheology of microcirculation. RBC aggregation is a major factor responsible for the flow properties of blood. Increased RBC aggregation has been observed in several pathological states. Therefore, the measurement of erythrocyte aggregation is rheologically important for quantifying flow abnormality in pathological conditions. Normal RBC under low flow or at rest form rouleaux aggregates, while abnormal RBC aggregation may lead to the formation of irregular aggregate structures, which may be induced by cell-associated factors (reduced membrane sialic acid levels) but also by extracellular factors. The main objective of the present investigation was to study RBC aggregate morphology in diabetic patients, using direct microscopic observation and numerical processing of recorded digitized images. Blood samples were obtained from 20 diabetic patients and from 15 normal control subjects. The aggregate morphology was quantified by the so-called Aggregate Shape Parameter (ASP) defined as the ratio of the aggregate projected area to its square perimeter. ASP appeared significantly higher (p < 10(-5)) in diabetic patients (0.65 +/- 0.18) than in normal controls (0.28 +/- 0.15). This rheo-optical method based on the theoretical model for rouleaux aggregates provides a useful reference for measuring deviations of RBC aggregate morphology. Increased aggregation of RBC resulting from a decreased sialylation of glycophorins may be an important factor in the development of vascular diseases and in the microcirculation impairment.
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Index: LILACS (Americas) Main subject: Diabetes Mellitus / Erythrocyte Aggregation / Erythrocytes Type of study: Etiology study / Observational study / Prognostic study Limits: Adult / Humans Language: Spanish Journal: Medicina (B.Aires) Journal subject: Medicine Year: 2000 Type: Article

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Index: LILACS (Americas) Main subject: Diabetes Mellitus / Erythrocyte Aggregation / Erythrocytes Type of study: Etiology study / Observational study / Prognostic study Limits: Adult / Humans Language: Spanish Journal: Medicina (B.Aires) Journal subject: Medicine Year: 2000 Type: Article