Aplicación de PCR-RFLP para subtipificar Campylobacter jejuni / [PCR-RFLP for Campylobacter jejuni subtyping]
Rev. argent. microbiol
;
37(2): 81-3, Apr.-June 2005.
Article
in Spanish
|
LILACS-Express
| LILACS, BINACIS
| ID: biblio-1171759
ABSTRACT
Ten Campylobacter jejuni isolates, 8 identified as C. jejuni biotype II of Lior and 2 as C. jejuni biotipe I, were recovered from aborted pig fetuses. In order to discriminate among strains, restriction fragment length polymorphism (RFLP) using Ddel of polymerase chain reaction (PCR) products of flaA gen was used. C. jejuni biotype II strains could be diferenciated in 6 by PCR-RFLP, and one subtype was obtained from C. jejunibiotype I. Although there is great variability of molecular techniques applied to the Campylobacter epidemiological studies, PCR-RFLP demonstrated to be a simple and accessible technique to discriminate Campylobacter jejuni isolates.
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Index:
LILACS (Americas)
Language:
Spanish
Journal:
Rev. argent. microbiol
Journal subject:
Microbiology
Year:
2005
Type:
Article
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