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LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat
Yin, Rong H; Wang, Yan R; Zhao, Su J; Yin, Rong L; Bai, Man; Wang, Ze Y; Zhu, Yu B; Cong, Yu Y; Liu, Hai Y; Bai, Wen L.
  • Yin, Rong H; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Wang, Yan R; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Zhao, Su J; Sichuan Animal Science Academy. College of Animal Science & Veterinary Medicine. Chengdu. CN
  • Yin, Rong L; Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin Province. Changchun. CN
  • Bai, Man; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Wang, Ze Y; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Zhu, Yu B; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Cong, Yu Y; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Liu, Hai Y; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
  • Bai, Wen L; Shenyang Agricultural University. College of Animal Science & Veterinary Medicine. Shenyang. CN
Electron. j. biotechnol ; 45: 19-29, May 15, 2020. tab, ilus, graf
Article in English | LILACS | ID: biblio-1177401
ABSTRACT

BACKGROUND:

Long non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown.

RESULTS:

In the present investigation, we verified that lncRNA-599554 had significantly higher expression at the anagen dermal papilla of cashmere goat SHF than that at telogen. Based on overexpression and knockdown techniques, we found that lncRNA-599554 contributes the inductive property of DPCs of cashmere goat, which was assessed by detecting the changes in the expression of several typical indictor genes in DPCs including ET-1, SCF, Versican, ALP, Lef1 and Ptc-1. Based on RNA pull-down assay, we verified that lncRNA-599554 directly interacted with chi-miR-15a-5p. Also, we showed that lncRNA-599554 positively regulated the Wnt3a expression in DPCs but which did not appear to involve its modulating of promoter methylation. Based on the use of Dual-luciferase reporter assays, our data indicated that lncRNA-599554 regulated the Wnt3a expression through chi-miR-15a-5p-mediated post-transcriptional level.

CONCLUSIONS:

We showed that lncRNA-599554 contributes the inductive property of DPCs in cashmere goat which might be achieved through sponging chi-miR-15b-5p to promote the Wnt3a expression. The results from the present investigation provided a novel insight into the functional mechanism of lncRNA-599554 in the SHF regeneration of cashmere goat along with the formation and growth of cashmere fiber.
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Full text: Available Index: LILACS (Americas) Main subject: Hair Follicle / Dermis / Wnt3A Protein / RNA, Long Noncoding Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin Province/CN / Shenyang Agricultural University/CN / Sichuan Animal Science Academy/CN

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Full text: Available Index: LILACS (Americas) Main subject: Hair Follicle / Dermis / Wnt3A Protein / RNA, Long Noncoding Limits: Animals Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Research Academy of Animal Husbandry and Veterinary Medicine Sciences of Jilin Province/CN / Shenyang Agricultural University/CN / Sichuan Animal Science Academy/CN