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VEGF and fgf-2 released in palatal suture after rapid maxillary expansion (RME)
Department of Clinical and Toxicological AnalysisStuani, Adriana Sasso; Department of OrthodonticSilvano, Paula Regina Ávila; Department of OrthodonticArnez, Maya Fernanda Manfrin; Department of OrthodonticMira, Paôla Caroline da Silva; Department of OrthodonticGorita, Maria Cecília; Department of OrthodonticMonteiro, Patrícia Maria; Department of OrthodonticMarsumoto, Mirian Aiko Nakame; Department of Clinical and Toxicological AnalysisHirata, Mario Hiroshi; Department of OrthodonticsMenezes, Luciane Macedo de; Department of OrthodonticStuani, Maria Bernadete Sasso.
  • Department of Clinical and Toxicological AnalysisStuani, Adriana Sasso; Universidade de São Paulo. School of Pharmaceutical Sciences. Department of Clinical and Toxicological AnalysisStuani, Adriana Sasso. São Paulo. BR
  • Department of OrthodonticSilvano, Paula Regina Ávila; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticSilvano, Paula Regina Ávila. Ribeirao Preto. BR
  • Department of OrthodonticArnez, Maya Fernanda Manfrin; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticArnez, Maya Fernanda Manfrin. Ribeirao Preto. BR
  • Department of OrthodonticMira, Paôla Caroline da Silva; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticMira, Paôla Caroline da Silva. Ribeirao Preto. BR
  • Department of OrthodonticGorita, Maria Cecília; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticGorita, Maria Cecília. Ribeirao Preto. BR
  • Department of OrthodonticMonteiro, Patrícia Maria; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticMonteiro, Patrícia Maria. Ribeirao Preto. BR
  • Department of OrthodonticMarsumoto, Mirian Aiko Nakame; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticMarsumoto, Mirian Aiko Nakame. Ribeirao Preto. BR
  • Department of Clinical and Toxicological AnalysisHirata, Mario Hiroshi; Universidade de São Paulo. School of Pharmaceutical Sciences. Department of Clinical and Toxicological AnalysisHirata, Mario Hiroshi. São Paulo. BR
  • Department of OrthodonticsMenezes, Luciane Macedo de; Pontifícia Universidade Católica do Rio Grande do Sul. School of Health and Life Sciences. Department of OrthodonticsMenezes, Luciane Macedo de. Porto Alegre. BR
  • Department of OrthodonticStuani, Maria Bernadete Sasso; Universidade de São Paulo. School of Dentistry of Ribeirao Preto. Department of OrthodonticStuani, Maria Bernadete Sasso. Ribeirao Preto. BR
Braz. dent. j ; 32(1): 98-103, Jan.-Feb. 2021. graf
Article in English | LILACS, BBO | ID: biblio-1180724
ABSTRACT
Abstract Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) have the ability to increase vascular proliferation and permeability. The aim of this study was to quantify the release of two diffusible angiogenic growth factors (VEGF and FGF-2) after rapid maxillary expansion (RME). Thirty animals were randomly assigned to two groups. Control group (5 rats - intact suture) and Experimental groups (25 rats with RME) which were evaluated in different periods of treatment. Five animals were euthanized in different periods of healing at 0, 1, 2, 3, 5 and 7 days after RME. RT-PCR was used to evaluate the gene expression of angiogenic growth factors released on different periods of study. Data were submitted to statistical analysis using ANOVA followed by Tukey test and significance was assumed at a=0.05. RT-PCR showed that mRNAs of VEGF and FGF-2 were expressed in intact palatal suture tissue. mRNAs of VEGF and FGF-2 was upregulated in early periods (24 h) after RME (p<0.001 and p<0.01, respectively). The molecular levels of VEGF never returned to its original baseline values, and FGF-2 expression decreased up to day 5 (p<0.001) and suddenly increased at day 7, returning to its original level. RME increased VEGF secretion, but decreased FGF-2 secretion when compared to intact tissue. The results showed that these angiogenic growth factors are released and regulated in the palatal suture tissue after RME and could make an important contribution to the knowledge of overall reparative response of the suture tissue during the bone remodeling process.
RESUMO
Resumo Fator de crescimento endothelial (VEGF) e fator de crescimento de fibroblasto (FGF-2) tem a capacidade de aumentar a proliferação e permeabilidade vascular. O objetivo deste estudo foi quantificar a liberação dos dois fatores de crescimento (VEGF e FGF-2) após expansão rápida da maxilla (ERM). Trinta animais foram divididos aleatoriamente em dois grupos. Grupo Controle (5 ratos - sutura intacta) e grupos Experimentais (25 ratos submetidos a ERM) que foram avaliados em períodos diferentes de tratamento. Cinco animais foram eutanaziados em diferentes períodos de avaliação aos 0, 2, 3, 5 e 7 dias após ERM. RT-PCR foi usado para avaliar a expressão gênica dos fatores de crescimento liberados nos diferentes períodos de estudo. Os dados foram submetidos à análise estatística usando ANOVA seguido do pós-teste de Tukey com nível de significância de a=0.05. RT-PCR mostrou que os RNAm de VEGF e FGF-2 estavam expressos na sutura palatina mediana intacta. Os RNAm de VEGF e FGF-2 foram estimulados nos períodos iniciais (24h) após ERM (p<0.001 e p<0.01, respectivamente). Os nívies moleculares de VEGF nunca retornaram aos valores originais, e a expressão de FGF-2 reduziu até o dia 5 (p<0.001) e de repente aumentou até o dia 7, retornando aos níveis originais. ERM aumentou a secreção de VEGF, mas diminuiu a secreção de FGF-2 quando comparado ao tecido intacto. Os resultados mostraram que estes fatores de crescimento são liberados e regulados na sutura palatina mediana após ERM e podem ser de importante contribuição para o entendimento da resposta reparadora geral do tecido da sutura durante o processo de remodelação óssea.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Fibroblast Growth Factor 2 / Palatal Expansion Technique Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Fibroblast Growth Factor 2 / Palatal Expansion Technique Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pontifícia Universidade Católica do Rio Grande do Sul/BR / Universidade de São Paulo/BR