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Comparison of Transforming Growth Factor 1 (TGF-β 1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation
Haq, Muhammad Zaal; Margono, Anggraini; Djauharie, R. A. H. Nilakesuma; Asrianti, Dini; Topanesa, Indra; Julianto, Indah.
  • Haq, Muhammad Zaal; Universitas Indonesia. Faculty of Dentistry. Conservative Dentistry Residency Program. Jakarta. ID
  • Margono, Anggraini; Universitas Indonesia. Faculty of Dentistry. Conservative Dentistry Department. Jakarta. ID
  • Djauharie, R. A. H. Nilakesuma; Universitas Sebelas Maret. Faculty of Medicine. Dermatology Department. Jakarta. ID
  • Asrianti, Dini; Universitas Sebelas Maret. Faculty of Medicine. Dermatology Department. Jakarta. ID
  • Topanesa, Indra; Universitas Sebelas Maret. Faculty of Medicine. Dermatology Department. Jakarta. ID
  • Julianto, Indah; Universitas Sebelas Maret. Faculty of Medicine. Dermatology Department. Jakarta. ID
Article in English | LILACS, BBO | ID: biblio-1180856
ABSTRACT
ABSTRACT

Objective:

To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and

Methods:

hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni's post hoc test with significant values (p<0.05).

Results:

Significant differences were noted in TGF-β1 expression between 1%, 5%, 10%, and 25% L-PRF and the control group (10% FBS). The highest TGF-β1 expression occurred with 25% L-PRF (0.645 ± 0.048), followed by 10% L-PRF (0.461 ± 0.035), 10% FBS (0.374 ± 0.013), 5% L-PRF (0.275 ± 0.045), and the lowest expression was with 1% L-PRF (0.160 ± 0.045).

Conclusion:

The best result of TGF-B1 expression in hDPSC differentiation was in the 25% L-PRF group.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Cell Culture Techniques / Culture Media / Dental Pulp / Platelet-Rich Fibrin Limits: Humans Country/Region as subject: Asia Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Indonesia Institution/Affiliation country: Universitas Indonesia/ID / Universitas Sebelas Maret/ID

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Full text: Available Index: LILACS (Americas) Main subject: Cell Culture Techniques / Culture Media / Dental Pulp / Platelet-Rich Fibrin Limits: Humans Country/Region as subject: Asia Language: English Journal: Pesqui. bras. odontopediatria clín. integr Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Indonesia Institution/Affiliation country: Universitas Indonesia/ID / Universitas Sebelas Maret/ID