Comparison of Transforming Growth Factor 1 (TGF-β 1) Expression in Various Lysate Platelet-Rich Fibrin (L-PRF) Concentrations on Human Dental Pulp Stem Cell Differentiation
Pesqui. bras. odontopediatria clín. integr
;
21: e5890, 2021. tab
Article
in English
| LILACS, BBO
| ID: biblio-1180856
ABSTRACT
ABSTRACT Objective:
To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material andMethods:
hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni's post hoc test with significant values (p<0.05).Results:
Significant differences were noted in TGF-β1 expression between 1%, 5%, 10%, and 25% L-PRF and the control group (10% FBS). The highest TGF-β1 expression occurred with 25% L-PRF (0.645 ± 0.048), followed by 10% L-PRF (0.461 ± 0.035), 10% FBS (0.374 ± 0.013), 5% L-PRF (0.275 ± 0.045), and the lowest expression was with 1% L-PRF (0.160 ± 0.045).Conclusion:
The best result of TGF-B1 expression in hDPSC differentiation was in the 25% L-PRF group.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cell Culture Techniques
/
Culture Media
/
Dental Pulp
/
Platelet-Rich Fibrin
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Indonesia
Institution/Affiliation country:
Universitas Indonesia/ID
/
Universitas Sebelas Maret/ID
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