Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G> A with Orofacial Cleft
Pesqui. bras. odontopediatria clín. integr
;
21: e5896, 2021. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-1180869
ABSTRACT
ABSTRACT Objective:
To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material andMethods:
A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test.Results:
The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group.Conclusion:
The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymorphism, Genetic
/
Genetic Variation
/
Cleft Lip
/
Cleft Palate
/
Methylenetetrahydrofolate Dehydrogenase (NADP)
Type of study:
Evaluation studies
/
Risk factors
Country/Region as subject:
Asia
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Indonesia
Institution/Affiliation country:
Universitas Indonesia/ID
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