Your browser doesn't support javascript.
loading
Expression of Vitamin D Receptor and Vitamin D Receptor Gene Polymorphisms (BsmI, FokI, and TaqI) in Patients with Pterygium / Expressão do gene do receptor da vitamina D e de seus polimorfismos (BsmI, FokI e TaqI) em pacientes com pterígio
Bilak, Semsettin; Çevik, Muhammer Özgür; Erdogdu, Ibrahim Halil; Bagis, Haydar.
  • Bilak, Semsettin; Adiyaman University. School of Medicine. Department of Ophthalmology. Adiyaman. TR
  • Çevik, Muhammer Özgür; Adiyaman University. School of Medicine. Department of Medical Genetics. Adiyaman. TR
  • Erdogdu, Ibrahim Halil; Adnan Menderes University. School of Medicine. Department of Pathology. Aydın. TR
  • Bagis, Haydar; Adiyaman University. School of Medicine. Department of Medical Genetics. Adiyaman. TR
Arq. bras. oftalmol ; 84(3): 241-248, May-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1248966
ABSTRACT
ABSTRACT

Purpose:

This study aimed to determine the role of vitamin D receptor in the pathogenesis of pterygium. The vitamin D receptor eexpression levels in pterygium tissue, blood vitamin D levels, and frequency of selected vitamin D receptor gene polymorphisms (BsmI, FokI, and TaqI) were compared between patients with pterygium and healthy participants.

Methods:

The study included patients with pterygiumeee (n=50) and healthy volunteers (n=50). The serum vitamin D levels were measured for both groups. Immunohistochemical staining for vitamin D receptor ewas performed on sections obtained from the pterygium and adjacent healthy conjunctival tissues of the same individuals. The genomic existence of vitamin D receptor epolymorphisms (BsmI, FokI, and TaqI) were analyzed in DNA obtained from venous blood of participants using polymerase chain reaction and restriction fragment length polymorphism methods.

Results:

There was no difference found between the serum vitamin D levels of patients with pterygium and healthy controls. However, tissue expression of vitamin D receptor was higher in the pterygium endothelial cells of micro-vessels (p=0.002), subepithelial stromal (p=0.04), and intravascular inflammatory cells (p=0.0001), in comparison with the adjacent healthy conjunctival tissue. Moreover, while the BBtt haplotype was 2-fold higher, the bbTt haplotype was 2.5-fold lower, and the BbTT haplotype was 2.25-fold lower in the control group than in the pterygium group (p<0.001).

Conclusions:

Vitamin D serum levels did not differ between the healthy and pterygium groups. Vitamin D receptor expression was increased in the pterygium tissue versus the adjacent healthy tissue. However, vitamin D receptor polymorphism analysis in patients with pterygium did not reveal any significant difference in BsmI, FokI, or TaqI polymorphisms in comparison with the healthy volunteers.(AU)
RESUMO
RESUMO

Objetivo:

Determinar o papel do receptor da vitamina D na patogênese do pterígio. Os níveis de expressão do receptor da vitamina D no tecido do pterígio, os níveis sanguíneos de vitamina D e a frequência de alguns polimorfismos do gene do receptor da vitamina D (BsmI, FokI e TaqI) foram comparados entre pacientes com pterígio e participantes saudáveis.

Métodos:

Foram incluídos pacientes com pterígio (n=50) e voluntários saudáveis (n=50). Os níveis séricos de vitamina D foram medidos em ambos os grupos. Foi feita uma coloração imuno-histoquímica para o receptor da vitamina D em cortes obtidos do pterígio e dos tecidos conjuntivais saudáveis adjacentes dos mesmos indivíduos. A existência de polimorfismos do receptor da vitamina D (BsmI, FokI e TaqI) no genoma foi analisada em DNA obtido do sangue venoso dos participantes, usando métodos de Polymerase chain reaction (PCR) e RFLP.

Resultados:

Não foi observada nenhuma diferença entre os níveis séricos de vitamina D dos pacientes com pterígio e os dos controles saudáveis. Entretanto, a expressão tissular do receptor da vitamina D foi maior nas células endoteliais dos microvasos do pterígio (p=0,002), nas células estromais sub-epiteliais (p=0,04) e nas células inflamatórias intravasculares (p=0,0001), quando comparada à expressão no tecido conjuntival saudável adjacente. Além disso, embora o haplótipo BBtt tenha sido duas vezes mais frequente, o haplótipo bbTt foi 2,5 vezes menos frequente e o haplótipo BbTT foi 2,25 vezes menos frequente no grupo de controle do que no grupo com pterígio (p<0,001).

Conclusões:

Os níveis séricos de vitamina D não apresentaram diferenças entre o grupo de pessoas saudáveis e o com pterígio. A expressão do receptor da vitamina D mostrou-se maior no grupo com pterígio do que no tecido saudável adjacente. Entretanto, a análise dos polimorfismos do receptor da vitamina D nos pacientes com pterígio não revelou qualquer diferença significativa nos polimorfismos BsmI, FokI ou TaqI em comparação com os voluntários saudáveis.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Vitamin D / Pterygium / Immunohistochemistry Type of study: Observational study / Prevalence study / Risk factors Limits: Humans Language: English Journal: Arq. bras. oftalmol Journal subject: Ophthalmology Year: 2021 Type: Article Affiliation country: Turkey Institution/Affiliation country: Adiyaman University/TR / Adnan Menderes University/TR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Vitamin D / Pterygium / Immunohistochemistry Type of study: Observational study / Prevalence study / Risk factors Limits: Humans Language: English Journal: Arq. bras. oftalmol Journal subject: Ophthalmology Year: 2021 Type: Article Affiliation country: Turkey Institution/Affiliation country: Adiyaman University/TR / Adnan Menderes University/TR