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Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation
Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Almeida, Tamires Cunha; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Oliveira, Mauro Lúcio Gonçalves de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dos; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando Medeiros; Souza, Gustavo Henrique Bianco de.
  • Amparo, Tatiane Roquete; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Rodrigues, Ivanildes Vasconcelos; Universidade Federal de Juiz de Fora. Departamento de Farmácia. Governador Valadares. BR
  • Seibert, Janaína Brandão; Universidade Federal de Ouro Preto. NUPEB. Ouro Preto. BR
  • Almeida, Tamires Cunha; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Cabral, Vivette Appolinário Rodrigues; Universidade Federal de Lavras. Departamento de Ciências Florestais. Lavras. BR
  • Vieira, Paula Melo de Abreu; Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Ouro Preto. BR
  • Brandão, Geraldo Célio; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Oliveira, Mauro Lúcio Gonçalves de; Instituto Mineiro de Agropecuária. Belo Horizonte. BR
  • Silva, Glenda Nicioli da; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Santos, Orlando David Henrique dos; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Vieira Filho, Sidney Augusto; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Teixeira, Luiz Fernando Medeiros; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
  • Souza, Gustavo Henrique Bianco de; Universidade Federal de Ouro Preto. CIPHARMA, Escola de Farmácia. Ouro Preto. BR
Braz. J. Pharm. Sci. (Online) ; 56: e18474, 2020. tab, graf
Article in English | LILACS | ID: biblio-1249171
Responsible library: BR40.1
Localization: BR40.1
ABSTRACT
Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics.
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Full text: Available Index: LILACS (Americas) Main subject: Plants, Medicinal / In Vitro Techniques / Plant Extracts / Catechin / Anti-Bacterial Agents Type of study: Prognostic study Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Mineiro de Agropecuária/BR / Universidade Federal de Juiz de Fora/BR / Universidade Federal de Lavras/BR / Universidade Federal de Ouro Preto/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plants, Medicinal / In Vitro Techniques / Plant Extracts / Catechin / Anti-Bacterial Agents Type of study: Prognostic study Language: English Journal: Braz. J. Pharm. Sci. (Online) Journal subject: Farmacologia / Terapˆutica / Toxicologia Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Mineiro de Agropecuária/BR / Universidade Federal de Juiz de Fora/BR / Universidade Federal de Lavras/BR / Universidade Federal de Ouro Preto/BR