Allium jesdianum Extract Induces Oxidative Stress and Necroptosis in Human Colorectal Cancer (HT-29) Cell Line
Braz. arch. biol. technol
;
64: e21200491, 2021. tab, graf
Article
in English
| LILACS
| ID: biblio-1249215
ABSTRACT
Abstract This study aimed to evaluate the toxic impact of hydro-alcoholic Allium jesdianum extract (AJE) on the growth of HT-29 human colorectal cancer cell line. Phytochemical analysis using gas chromatography and mass spectroscopy (GCMS) was done to determine the bioactive components of AJE. HT-29 cells exposed to 0 (control), 25, 50, and 100 g/mL of AJE for 48 hours. Cell survival, colony numbers, flow cytometry, oxidative stress, and gene expression were examined to evaluate the toxic impacts of the AJE. Twelve different phyto-constituents with peak areas were determined by the GCMS analysis. The major compounds were Allicin and α-Pinene. AJE considerably reduced the viability and colony numbers of the HT-29 cells. The AJE concentration-dependently increased necrosis, but not apoptosis in the HT-29 cells. AJE upregulated the expression of necroptosis-associated genes including RIPK1, RIPK3, and MLKL in a concentration-dependent manner. AJE also dose-dependently enhanced MDA contents and reactive oxygen species (ROS) level and diminished antioxidant enzyme level in the HT-29 cells. These data collectively indicated that AJE prevented the growth of the HT-29 cells by inducing oxidative stress, and activation necroptosis signaling pathways.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Allium
/
Colorectal Neoplasms
/
Oxidative Stress
/
Necroptosis
Limits:
Humans
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2021
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Ahvaz Jundishapur University of Medical Sciences/IR
/
Legal Medicine Organization/IR
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