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Transcriptome comparison of dengue-susceptible and -resistant field derived strains of Colombian Aedes aegypti using RNA-sequencing
C2D2 Research GroupCoatsworth, Heather; Caicedo, Paola A; Winsor, Geoffrey; Brinkman, Fiona; Ocampo, Clara B; C2D2 Research GroupLowenberger, Carl.
  • C2D2 Research GroupCoatsworth, Heather; Simon Fraser University. Department of Biological Sciences. C2D2 Research GroupCoatsworth, Heather. Burnaby. CA
  • Caicedo, Paola A; Universidad Icesi. Natural Science Faculty. Centro Internacional de Entrenamiento e Investigaciones Médicas, Department of Biology, Vector Biology and Control. Cali. CO
  • Winsor, Geoffrey; Simon Fraser University. Department of Molecular Biology and Biochemistry. Burnaby. CA
  • Brinkman, Fiona; Simon Fraser University. Department of Molecular Biology and Biochemistry. Burnaby. CA
  • Ocampo, Clara B; Universidad Icesi. Natural Science Faculty. Centro Internacional de Entrenamiento e Investigaciones Médicas, Department of Biology, Vector Biology and Control. Cali. CO
  • C2D2 Research GroupLowenberger, Carl; Simon Fraser University. Department of Biological Sciences. C2D2 Research GroupLowenberger, Carl. Burnaby. CA
Mem. Inst. Oswaldo Cruz ; 116: e200547, 2021. tab, graf
Article in English | LILACS | ID: biblio-1250365
ABSTRACT
BACKGROUND Forty percent of the world's population live in areas where they are at risk from dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Dengue viruses are transmitted primarily by the mosquito Aedes aegypti. In Cali, Colombia, approximately 30% of field collected Ae. aegypti are naturally refractory to all four dengue serotypes. OBJECTIVES Use RNA-sequencing to identify those genes that determine refractoriness in feral mosquitoes to dengue. This information can be used in gene editing strategies to reduce dengue transmission. METHODS We employed a full factorial design, analyzing differential gene expression across time (24, 36 and 48 h post bloodmeal), feeding treatment (blood or blood + dengue-2) and strain (susceptible or refractory). Sequences were aligned to the reference Ae. aegypti genome for identification, assembled to visualize transcript structure, and analyzed for dynamic gene expression changes. A variety of clustering techniques was used to identify the differentially expressed genes. FINDINGS We identified a subset of genes that likely assist dengue entry and replication in susceptible mosquitoes and contribute to vector competence. MAIN CONCLUSIONS The differential expression of specific genes by refractory and susceptible mosquitoes could determine the phenotype, and may be used to in gene editing strategies to reduce dengue transmission.
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Full text: Available Index: LILACS (Americas) Main subject: Aedes / Dengue / Dengue Virus Type of study: Prognostic study Limits: Animals Country/Region as subject: South America / Colombia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2021 Type: Article Affiliation country: Canada / Colombia Institution/Affiliation country: Simon Fraser University/CA / Universidad Icesi/CO

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Full text: Available Index: LILACS (Americas) Main subject: Aedes / Dengue / Dengue Virus Type of study: Prognostic study Limits: Animals Country/Region as subject: South America / Colombia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2021 Type: Article Affiliation country: Canada / Colombia Institution/Affiliation country: Simon Fraser University/CA / Universidad Icesi/CO