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Chemical composition, antimicrobial and antifungal activity of Lippia Thymoide essential oil in oral pathogens
Carvalho, Tabata Resque Beckmann; Tanaka, Erich Brito; Vilhena, Amujacy Tavares; Frade, Paula Cristina Rodrigues; Fonseca, Ricardo Roberto de Souza; Rodrigues, Tânia Maria de Souza; Brito, Mileide da Paz; Silva, Sebastião Gomes; Carvalho Junior, Raul Nunes de; Oliveira, Mozaniel Santana de; Andrade, Eloisa Helena de Aguiar; Oliveira-Filho, Aldemir Branco; Menezes, Silvio Augusto Fernandes de.
  • Carvalho, Tabata Resque Beckmann; University Center of State of Pará. School of Dentistry. Department of periodontology. Belém. BR
  • Tanaka, Erich Brito; University Center of State of Pará. School of Dentistry. Department of periodontology. Belém. BR
  • Vilhena, Amujacy Tavares; University Center of State of Pará. School of Dentistry. Department of periodontology. Belém. BR
  • Frade, Paula Cristina Rodrigues; Federal University of Pará. Tropical Medicine Nucleus. Belém. BR
  • Fonseca, Ricardo Roberto de Souza; Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém. BR
  • Rodrigues, Tânia Maria de Souza; University Center of State of Pará. School of Dentistry. Department of periodontology. Belém. BR
  • Brito, Mileide da Paz; University Center of State of Pará. School of Pharmacy. Department of pharmaceutics. Belém. BR
  • Silva, Sebastião Gomes; Federal University of Pará. Program of Post-Graduation in Chemistry. Belém. BR
  • Carvalho Junior, Raul Nunes de; Federal University of Pará. Faculty of Food Engineering (LABEX/FEA). Program of Post-Graduation in Food Science and Technology. Belém. BR
  • Oliveira, Mozaniel Santana de; Federal University of Pará. Faculty of Food Engineering (LABEX/FEA). Program of Post-Graduation in Food Science and Technology. Belém. BR
  • Andrade, Eloisa Helena de Aguiar; Museu Paraense Emílio Goeldi. Adolpho Ducke Laboratory. Belém. BR
  • Oliveira-Filho, Aldemir Branco; Federal University of Pará. Institute for Coastal Studies. Study and Research Group on Vulnerable Populations. Bragança. BR
  • Menezes, Silvio Augusto Fernandes de; University Center of State of Pará. School of Dentistry. Department of periodontology. Belém. BR
Braz. j. oral sci ; 20: e210219, jan.-dez. 2021. ilus
Article in English | BBO, LILACS | ID: biblio-1253954
ABSTRACT

Aim:

This study evaluated the chemical composition of Lippia thymoides (Lt) essential oil and its antimicrobial activity against fungal strains of Candida albicans (Ca) and Gram-negative bacteria Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn).

Methods:

Lt essential oil was obtained by hydrodistillation apparatus with a modified Clevenger extension. The chemical analysis was analyzed by gas phase chromatography and mass spectrometry on Shimadzu QP 2010 plus. Sample sensitivity evaluation was performed by ABHb-inoculum and culture plates were developed with triphenyltetrazolium chloride, also Fn and Pi samples analysis were in anaerobic environment and Ca sample analysis was performed in aerobic environment. The minimum inhibitory concentration (CIM) was determinated by microdilution in eppendorfs tubes.

Results:

The chemical analysis showed that Thymol (59,91%) is the main compound found in Lt essential oil, also other antifungal and antimicrobial agents were present γ-terpinene (8.16%), p-cymene (7.29%) and ß-caryophyllene (4.49%), Thymol is a central ingredient of many medicinal plants and has a potent fungicidal, bactericidal and antioxidant activity, it has been previously shown to have anti-inflammatory activity against Periodontal Disease (PD) cause can reduces prostanoids, interleukins, leukotrienes levels in periodontium. CIM result Pi was 6.5 µg/mL, Fn was 1.5 µg/mL and Ca was 0.19 µg/mL.

Conclusion:

The antimicrobial activity of L. thymoides, through the compound Thymol, has been shown promising potential against gram-negative periodontopathogenic bacteria and fungi whose therapeutic arsenal is still very restricted
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Oils, Volatile / Plant Extracts / Lippia / Microbiota / Antifungal Agents Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Pará/BR / Museu Paraense Emílio Goeldi/BR / University Center of State of Pará/BR

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Full text: Available Index: LILACS (Americas) Main subject: Periodontitis / Oils, Volatile / Plant Extracts / Lippia / Microbiota / Antifungal Agents Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Pará/BR / Museu Paraense Emílio Goeldi/BR / University Center of State of Pará/BR