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Improving micropropagation of some grape cultivars via boron, calcium and phosphate
Al-Aizari, Ahmed Ali; Al-Obeed, Rashid S; Mohamed, Mahmoud AH.
  • Al-Aizari, Ahmed Ali; King Saud University. College of Food and Agricultural Sciences. Department of Plant Production. Riyadh. SA
  • Al-Obeed, Rashid S; King Saud University. College of Food and Agricultural Sciences. Department of Plant Production. Riyadh. SA
  • Mohamed, Mahmoud AH; Minia University. Faculty of Agriculture. Department of Horticulture. EG
Electron. j. biotechnol ; 48: 95-100, nov. 2020. ilus, tab
Article in English | LILACS | ID: biblio-1254838
ABSTRACT

BACKGROUND:

An efficient regeneration protocol is a priority for the successful application of plant biotechnology. Grape nodal explants were used to develop a micropropagation protocol for Thompson Seedless and Taify cvs. Explants were cultured on MS medium supplemented with Kinetin or benzylaminopurine (BA) and indolebutyric acid (IBA).

RESULTS:

For both cultivars, axillary buds were grown, only, on a medium enriched with kinetin, moreover, shoot tip necrosis and callus formation were observed on Thompson Seedless cv. cultures grown on a medium with BA. Supplementing the growth medium with 100 mM (boron) B and 2.5 mM (calcium) Ca successfully help overcome these phenomena. The highest regenerated shoot numbers (14 and 6.2 explant 1 ) for Taify and Thompson Seedless cvs., respectively, were on media supplemented with 13.2 mM BA + 4.9 mM IBA and BA 13.2 mM + 5.8 mM IBA, respectively. Moreover, these media supported the developing shoots to have the heaviest dry weights (1.46 and 0.72 mg explant 1 ) for Taify and Thompson Seedless cvs., respectively. Thompson Seedless cv. regenerated shoot numbers and their dry weights were significantly increased by increasing the MS medium PO4 concentration. However, these two parameters were significantly decreased for Taify cv. Developing shoots were elongated and rooted on MS medium enriched with 4.9 mM, IBA 100 mM B and 2.5 mM Ca. Plantlets were acclimatized and successfully transferred to the greenhouse conditions.

CONCLUSIONS:

A novel promising protocol for Thomson Seedless and Taify cvs. micropropagation using single nodes has been developed.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Phosphates / Boron / Calcium / Vitis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: King Saud University/SA / Minia University/EG

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Full text: Available Index: LILACS (Americas) Main subject: Phosphates / Boron / Calcium / Vitis Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: King Saud University/SA / Minia University/EG