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Protective effect of N-acetyl-L-cysteine and rosuvastatin against oxidative stress in fibroblasts from asymptomatic patients with X-ALD: a preliminary study
Marchetti, Desirèe; Ribas, Graziela; Donida, Bruna; Brito, Verônica; Moura, Dinara; Coelho, Daniella; Machado, Andryele; Kessler, Rejane; Coitinho, Adriana; Vargas, Carmen.
  • Marchetti, Desirèe; Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Programa de Graduação em Ciências Biológicas. Porto Alegre. BR
  • Ribas, Graziela; Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre. BR
  • Donida, Bruna; Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Programa de Graduação em Ciências Biológicas. Porto Alegre. BR
  • Brito, Verônica; Universidade Federal de Ciências da Saúde de Porto Alegre. Porto Alegre. BR
  • Moura, Dinara; Universidade Federal de Ciências da Saúde de Porto Alegre. Porto Alegre. BR
  • Coelho, Daniella; Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre. BR
  • Machado, Andryele; Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre. BR
  • Kessler, Rejane; Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. Porto Alegre. BR
  • Coitinho, Adriana; Universidade Federal do Rio Grande do Sul. Instituto de Ciências da Saúde Básica. Porto Alegre. BR
  • Vargas, Carmen; Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Programa de Graduação em Ciências Biológicas. BR
Clin. biomed. res ; 41(1): 57-64, 2021. tab, graf
Article in English | LILACS | ID: biblio-1255192
ABSTRACT

Introduction:

Several studies in the literature have evaluated the role of oxidative stress and adjuvant therapies for X-linked adrenoleukodystrophy (X-ALD). Here, we investigated whether n-acetyl-L-cysteine (NAC) and rosuvastatin (RSV) could influence the generation of reactive species, redox status and nitrative stress in fibroblasts from asymptomatic patients with X-ALD.

Methods:

Skin biopsy samples were cultured and treated for 2 hours (37 °C) with NAC and RSV.

Results:

X-ALD fibroblasts generated high levels of reactive oxygen species. These levels were significantly lower in fibroblasts treated with NAC and RSV relative to untreated samples. The X-ALD fibroblasts from asymptomatic patients also had higher catalase activity, and only NAC was able to increase enzyme activity in the samples.

Conclusions:

Our results indicated that NAC and RSV were able to improve oxidative stress parameters in fibroblasts from asymptomatic patients with X-ALD, showing that adjuvant antioxidant therapy may be a promising treatment strategy for asymptomatic patients with this disease. (AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Acetylcysteine / Oxidative Stress / Adrenoleukodystrophy / Rosuvastatin Calcium Limits: Female / Humans / Male Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital de Clínicas de Porto Alegre/BR / Universidade Federal de Ciências da Saúde de Porto Alegre/BR / Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Acetylcysteine / Oxidative Stress / Adrenoleukodystrophy / Rosuvastatin Calcium Limits: Female / Humans / Male Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital de Clínicas de Porto Alegre/BR / Universidade Federal de Ciências da Saúde de Porto Alegre/BR / Universidade Federal do Rio Grande do Sul/BR