Molecular epidemiology of hepatitis C virus (HCV) in Kadun State
Afr. J. Clin. Exp. Microbiol
; 13(3): 61-65, 2012. ilus
Article
in En
| AIM
| ID: biblio-1256064
Responsible library:
CG1.1
ABSTRACT
Objective:
To determine the distribution of hepatitis C virus (HCV) genotypes and subtypes among blood donors and outpatients attendees positive for antibody to HCV (anti-HCV). Justification Hepatitis C virus (HCV) continues to be a major disease burden on the world and Man is the only known natural host of Hepatitis C virus (Chivaliez and Pawlotsky; 2007). There is no published data on the prevalence of the genotypes and subtypes of HCV in Kaduna State.Setting:
Three hospitals one in each of the 3 senatorial zones in Kaduna State. Patients Blood donors who reported for blood donation and outpatient department attendees.Method:
Antibody detection by a third generation HCV ELISA (Biotech Laboratories; UK); HCV RNA and genotyping by Reverse Transcriptase polymerase chain reaction with genotype-specific primers. (Sacace Biotechnologies; UK).Results:
of the 259 plasma specimens screened for Hepatitis C virus in this study; 20(7.7) were positive for anti-HCV antibodies by ELISA and 16(6.2) of the antibodies positive specimen were positive for HCV RNA. Of the 139 blood donors tested; 8 (5.8) were HCV RNA positive. Similarly; 120 were tested from the outpatient Department attendees and 8 (6.7) were HCV RNA positive. Hepatitis C virus genotype 1b was found in the entire HCV RNA positive sample.Conclusions:
The findings of 6.2prevalence of HCV infection based on HCV RNA test confirmed that there is Hepatitis C virus in Kaduna State with genotype 1b as the predominant genotype found in all the three senatorial zones
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Index:
AIM
Main subject:
Bandages
/
Wound Infection
/
HIV Infections
/
Molecular Epidemiology
/
Hepacivirus
/
Nigeria
Type of study:
Risk_factors_studies
/
Screening_studies
Country/Region as subject:
Africa
Language:
En
Journal:
Afr. J. Clin. Exp. Microbiol
Year:
2012
Type:
Article