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Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
Liu, Feng; Ruan, Yingying.
  • Liu, Feng; Ningbo City College of Vocational Technology. Department of Horticultural Technology. Ningbo City. CN
  • Ruan, Yingying; Ningbo University. Department of Biotechnology. Ningbo City. CN
Braz. arch. biol. technol ; 64: e21200316, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278451
ABSTRACT
Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Plants, Genetically Modified / Arabidopsis / Fragaria / Herbicides Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Ningbo City College of Vocational Technology/CN / Ningbo University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Plants, Genetically Modified / Arabidopsis / Fragaria / Herbicides Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Ningbo City College of Vocational Technology/CN / Ningbo University/CN