Biological and Oxidative Effect of Ellagic Acid on Saccharomyces cerevisiae: A New Way for Culture Developing
Braz. arch. biol. technol
; 64: e21210002, 2021. tab, graf
Article
in En
| LILACS
| ID: biblio-1278453
Responsible library:
BR1.1
ABSTRACT
Abstract In this study, the effects of Ellagic acid (EA) on protein expression in yeasts and cellular development were investigated. Four groups were formed. Groups 1) Control group; yeast only cultivated group; 2) Ellagic Acid (EA) group EA (10%) given group; 3) Hydrogen peroxide (H2O2) Group The group given H2O2 (15 mM); 4) EA + H2O2 group EA (10%) + H2O2 (15 mM) group. After sterilization, EA (10%) and H2O2 (15 mM) were added to the Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours (overnight). S. cerevisiae cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) level were analyzed by spectrophotometer. Total protein changes were determined by SDS-PAGE electrophoresis and measured by the Bradford method. According to the obtained results, compared with the H2O2 group, cell development (1, 3, 5 and 24 hours), GSH level and total protein synthesis (24 hours) were increased with EA, while MDA level (24 hours) decreased. These results show that EA reduces oxidative damage, increases cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture.
Key words
Full text:
1
Index:
LILACS
Main subject:
Saccharomyces cerevisiae
/
Electrophoresis, Polyacrylamide Gel
/
Ellagic Acid
/
Hydrogen Peroxide
Limits:
Humans
Language:
En
Journal:
Braz. arch. biol. technol
Journal subject:
BIOLOGIA
Year:
2021
Type:
Article