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Evaluation of muscle involvement & degeneration in advanced oral submucous fibrosis using three different stains ­ a retrospective cross-sectional study / Avaliação do envolvimento e degeneração muscular na fibrose submucosa oral avançada usando três colorações diferentes - um estudo transversal retrospectivo
Umashankar, Krishnapriya; Sherlin, Herald; Jayaraj, Gifrina; Don, Kanchi Ravi; Santhanam, Archana.
  • Umashankar, Krishnapriya; Saveetha Institute of Medical and Technical Sciences. Saveetha Dental College and Hospital. Department of Oral Pathology. Tamil Nadu. IN
  • Sherlin, Herald; Saveetha Institute of Medical and Technical Sciences. Saveetha Dental college and Hospital. Department of Oral Pathology. Tamil Nadu. IN
  • Jayaraj, Gifrina; Saveetha Institute of Medical and Technical Sciences. Saveetha dental College and Hospital. Department of Oral Pathology. Tamil Nadu. IN
  • Don, Kanchi Ravi; Saveetha Institute of Medical and Technical Sciences. Saveetha Dental College and Hospital. Department of Oral Pathology. Tamil Nadu. IN
  • Santhanam, Archana; Saveetha Institute of Medical and Technical Sciences. Saveetha Dental College and Hospital. Department of Oral Pathology. Tamil Nadu. IN
Braz. dent. sci ; 24(3): 1-7, 2021. tab, ilus
Article in English | BBO, LILACS | ID: biblio-1281389
ABSTRACT

Background:

Oral submucous fibrosis (OSMF) is graded according to various histological factors which include the epithelial changes and the connective tissue changes. These features could be identified in routine Hematoxylin and Eosin (H and E) staining in shades of pink. However, the use of special stains provides a contrast to various connective tissue components thereby aiding in better visualization of these connective tissue changes in advanced OSMF cases.

Objective:

To compare and evaluate muscle involvement and degeneration in advanced oral submucous fibrosis using three different stains namely, H&E, Van Gieson, and Masson's Trichrome. Material and

Methods:

10 Formalin-fixed paraffin-embedded tissue sections of advanced OSMFwere stained using 3 different stains namely Hematoxylin and Eosin (H&E), Van-Gieson, and Masson trichrome. The results obtained were tabulated and statistically analyzed using Kruskal Wallis ANOVA.

Results:

The hyalinization and fibrosis involving the skeletal muscle were better visualized in Masson's Trichrome but were not statistically significant. The muscle degeneration in deeper areas was better visualized in Masson's trichrome when compared to the H&E and Van Gieson.

Conclusion:

Hematoxylin and eosin stains all the connective tissue components in various shades of pink, use of special stains bestows contrast between different components of connective tissue, thus improvising grading of OSMF. Masson's trichrome stain can be used as a single adjunct to hematoxylin and eosin stain as changes in the superficial and deeper connective tissue could be ascertained (AU)
RESUMO

Contexto:

A fibrose submucosa oral (FSO) é classificada de acordo com vários fatores histológicos que incluem alterações epiteliais e do tecido conjuntivo. Essas características podem ser identificadas na coloração de rotina com Hematoxilina e Eosina (H&E) em tons de rosa. No entanto, o uso de colorações especiais fornece um contraste para vários componentes do tecido conjuntivo, auxiliando assim na melhor visualização dessas alterações do tecido conjuntivo em casos avançados de FSO.

Objetivo:

Comparar e avaliar o envolvimento e degeneração muscular da fibrose submucosa oral avançada usando três colorações diferentes H&E, Van Gieson e Tricrômio de Masson. Material e

Métodos:

10 seções de tecido fixadas em formalina e embebidas em parafina de FSO avançada foram coradas usando essas três colorações. Os resultados obtidos foram tabulados e analisados estatisticamente por meio do teste de Kruskal Wallis.

Resultados:

A hialinização e fibrose envolvendo o músculo esquelético foram melhor visualizadas no tricromo de Masson, mas não foram estatisticamente significativas. A degeneração muscular em áreas mais profundas foi melhor visualizada no tricrômico de Masson, quando comparado ao H&E e Van Gieson.

Conclusão:

Hematoxilina e Eosina coloram todos os componentes do tecido conjuntivo em vários tons de rosa. O uso de colorações especiais confere contraste entre os diferentes componentes do tecido conjuntivo, melhorando assim a avaliação da FSO. A coloração com tricrômio de Masson pode ser usada como um único adjunto para a coloração de Hematoxilina e Eosina, pois assim é possível verificar alterações superficiais e profundas no tecido conjuntivo. (AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oral Submucous Fibrosis / Fibrosis / Connective Tissue Type of study: Observational study / Prevalence study / Prognostic study Language: English Journal: Braz. dent. sci Journal subject: Dentistry / Sa£de Bucal Year: 2021 Type: Article Affiliation country: India Institution/Affiliation country: Saveetha Institute of Medical and Technical Sciences/IN

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Full text: Available Index: LILACS (Americas) Main subject: Oral Submucous Fibrosis / Fibrosis / Connective Tissue Type of study: Observational study / Prevalence study / Prognostic study Language: English Journal: Braz. dent. sci Journal subject: Dentistry / Sa£de Bucal Year: 2021 Type: Article Affiliation country: India Institution/Affiliation country: Saveetha Institute of Medical and Technical Sciences/IN