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Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil
Mahdi, Salih Abdul; Ali Kadhim, Afraa; Albukhaty, Salim; Nikzad, Safoora; Haider, Adawiya J; Ibraheem, Sumayah; Ali Kadhim, Haitham; Al-Musawi, Sharafaldin.
  • Mahdi, Salih Abdul; Al-Qasim Green University. College of Biotechnology. Babylon. IQ
  • Ali Kadhim, Afraa; Mustansiriyah University. College of Science. Department of Biology. Baghdad. IQ
  • Albukhaty, Salim; University of Misan. College of Science. Department of Chemistry. Maysan. IQ
  • Nikzad, Safoora; Hamadan University of Medical Science. Faculty of Medicine. Department of Medical Physics. Hamadan. IR
  • Haider, Adawiya J; University of Technology. Applied Science Department/Laser Science and Technology Branch. Bagdad. IQ
  • Ibraheem, Sumayah; Al_kindy Medicine College. Bagdad. IQ
  • Ali Kadhim, Haitham; Iraq Ministry of Health. Medico Legal Directorate. Bagdad. IQ
  • Al-Musawi, Sharafaldin; Al-Qasim Green University. College of Biotechnology. Babylon. IQ
Electron. j. biotechnol ; 52: 21-29, July. 2021. ilus, tab, graf
Article in English | LILACS | ID: biblio-1283484
Responsible library: CL1.1
ABSTRACT

BACKGROUND:

Super-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.

RESULTS:

In this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEXSPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FUSPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and 45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.

CONCLUSIONS:

The findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / Fluorouracil / Liver Neoplasms Limits: Humans Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: Iran / Iraq Institution/Affiliation country: Al-Qasim Green University/IQ / Al_kindy Medicine College/IQ / Hamadan University of Medical Science/IR / Iraq Ministry of Health/IQ / Mustansiriyah University/IQ / University of Misan/IQ / University of Technology/IQ

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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / Fluorouracil / Liver Neoplasms Limits: Humans Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: Iran / Iraq Institution/Affiliation country: Al-Qasim Green University/IQ / Al_kindy Medicine College/IQ / Hamadan University of Medical Science/IR / Iraq Ministry of Health/IQ / Mustansiriyah University/IQ / University of Misan/IQ / University of Technology/IQ