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Amplification of urea detection based on pH-sensitive liposomes
Kyeong Kang, Min; Park, Jin-Won.
  • Kyeong Kang, Min; Seoul National University of Science and Technology. College of Energy and Biotechnology. Department of Chemical and Biomolecular Engineering. Seoul. KR
  • Park, Jin-Won; Seoul National University of Science and Technology. College of Energy and Biotechnology. Department of Chemical and Biomolecular Engineering. Seoul. KR
Electron. j. biotechnol ; 52: 30-34, July. 2021. ilus, tab, graf
Article in English | LILACS | ID: biblio-1283487
Responsible library: CL1.1
ABSTRACT

BACKGROUND:

This study aimed to develop an amplification method of urea detection based on pHsensitive liposomes.

RESULTS:

The urease covalently immobilized on the magnetic particles and the pH-sensitive liposomes encapsulating ferricyanide were added to the cyclic-voltammeter cell solution where urea was distributed. The conversion of urea into carbonic acid seemed to induce a pH decrease that caused a reduction in the electrostatic repulsion between the headgroups of weakly acidic 1,2-dipalmitoyl-sn-glycero3-succinate. The reduction induced the liposomes to release potassium ferricyanide that was encapsulated inside. The effects of urea concentration and pH value were investigated. A specific concentration (0.5 mg/mL) of the urea solution was set to observe the response. The activity of urease was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to the comparable techniques such as an enzyme-linked immunosorbent assay and a field-effect transistor.

CONCLUSIONS:

In summary, the methodology developed in this study was feasible as a portable, rapid, and sensitive method.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Urea / Liposomes Type of study: Diagnostic study Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: South Korea Institution/Affiliation country: Seoul National University of Science and Technology/KR

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Full text: Available Index: LILACS (Americas) Main subject: Urea / Liposomes Type of study: Diagnostic study Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: South Korea Institution/Affiliation country: Seoul National University of Science and Technology/KR