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Patrón EPIYA en cepas de Helicobacter pylori CagA positivas en pacientes del Hospital Regional de Talca / EPIYA pattern in Helicobacter pylori CagA positive strains in patients of the Regional Hospital of Talca
González Bonet, Ileana; Romero Elías, María Jacqueline; Morales Mejías, Erik; Valdez Moyano, Eliana; Cofré Loyola, Cecilia; Manques Maldonado, Belarmino; Rojas Rubio, Armando.
  • González Bonet, Ileana; Universidad Católica del Maule. Facultad de Medicina. Laboratorios de Investigaciones Biomédicas. CL
  • Romero Elías, María Jacqueline; Universidad Católica del Maule. Facultad de Medicina. Laboratorios de Investigaciones Biomédicas. CL
  • Morales Mejías, Erik; Universidad Católica del Maule. Facultad de Medicina. Laboratorios de Investigaciones Biomédicas. CL
  • Valdez Moyano, Eliana; Universidad Católica del Maule. Facultad de Medicina. Departamento de Ciencias Clínicas. Talca. CL
  • Cofré Loyola, Cecilia; Universidad Católica del Maule. Facultad de Medicina. Departamento de Ciencias Clínicas. Talca. CL
  • Manques Maldonado, Belarmino; Universidad Católica del Maule. Facultad de Medicina. Departamento de Ciencias Clínicas. Talca. CL
  • Rojas Rubio, Armando; Universidad Católica del Maule. Facultad de Medicina. Laboratorios de Investigaciones Biomédicas. CL
Rev. méd. Maule ; 33(1): 8-13, jun. 2017. tab
Article in Spanish | LILACS | ID: biblio-1283791
ABSTRACT

BACKGROUND:

The clinical outcome of Helicobacter pylori (H. pylori) infection has been related to the presence of CagA protein. This protein is highly polymorphic and its oncogenic ability depends on the number and type of tyrosine phosphorylation sites in the EPIYAs repeat sequences (A, B, C and D).

AIM:

To determine the EPIYA patterns of the CagA gene in H. pylori strains and its relationship with gastrointestinal pathology in infected patients of the Regional Hospital of Talca. MATERIAL AND

METHODS:

The strains were isolated from gastric biopsies and characterized by bacteriological and molecular methods. Gastrointestinal pathology was characterized by histopathological analysis. For the determination of the presence of the cagA gene and the EPIYAs standards, the conventional PCR technique was used.

RESULTS:

138 DNA samples from H. pylori strains were analyzed. 92.0% (127/138) of the isolates carried the cagA gene, of which 66 (52.0%) corresponded to the EPIYA-ABC pattern, 43 (33.8%) to the EPIYA-ABCC pattern and 21 16.5%) to the EPIYA-ABCCC phosphorylation pattern. 50.4% (64/127) of cagA positive strains isolated from dyspeptic patients in the Maule region have more than two C sites of phosphorylation. The number of EPIYAs C motifs was associated with the presence of more severe histopathological damage in the gastric mucosa.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Stomach Neoplasms / Helicobacter pylori / Helicobacter Infections / Amino Acid Motifs Type of study: Practice guideline Limits: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male Country/Region as subject: South America / Chile Language: Spanish Journal: Rev. méd. Maule Journal subject: Medicine Year: 2017 Type: Article Affiliation country: Chile Institution/Affiliation country: Universidad Católica del Maule/CL

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Full text: Available Index: LILACS (Americas) Main subject: Stomach Neoplasms / Helicobacter pylori / Helicobacter Infections / Amino Acid Motifs Type of study: Practice guideline Limits: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male Country/Region as subject: South America / Chile Language: Spanish Journal: Rev. méd. Maule Journal subject: Medicine Year: 2017 Type: Article Affiliation country: Chile Institution/Affiliation country: Universidad Católica del Maule/CL