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Effects of the lower energy and pulse stacking in carbon dioxide laser skin treatment: an objective analysis using second harmonic generation
Department of SurgeryMotta, Marcos Matias; Department of Anatomic PathologyStelini, Rafael Fantelli; Department of SurgeryCalderoni, Davi Reis; Multidisciplinary Center for Biological Investigation on Laboratory Animals ScienceGilioli, Rovilson; Damiani, Gislaine Vieira; Biophotonics LaboratoryCésar, Carlos Lenz; Department of SurgeryKharmandayan, Paulo.
  • Department of SurgeryMotta, Marcos Matias; Universidade Estadual de Campinas. Faculty of Medical Sciences. Department of SurgeryMotta, Marcos Matias. Campinas. BR
  • Department of Anatomic PathologyStelini, Rafael Fantelli; Universidade Estadual de Campinas. Faculty of Medical Sciences. Department of Anatomic PathologyStelini, Rafael Fantelli. Campinas. BR
  • Department of SurgeryCalderoni, Davi Reis; Universidade Estadual de Campinas. Faculty of Medical Sciences. Department of SurgeryCalderoni, Davi Reis. Campinas. BR
  • Multidisciplinary Center for Biological Investigation on Laboratory Animals ScienceGilioli, Rovilson; Universidade Estadual de Campinas. Faculty of Medical Sciences. Multidisciplinary Center for Biological Investigation on Laboratory Animals ScienceGilioli, Rovilson. Campinas. BR
  • Damiani, Gislaine Vieira; Instituto Federal de Educação, Ciência e Tecnologia de São Paulo. Biophotonics Laboratory. Capivari. BR
  • Biophotonics LaboratoryCésar, Carlos Lenz; Universidade Estadual de Campinas. National Institute of Photonics Applied to Cell Biology. Biophotonics LaboratoryCésar, Carlos Lenz. Campinas. BR
  • Department of SurgeryKharmandayan, Paulo; Universidade Estadual de Campinas. Faculty of Medical Sciences. Department of SurgeryKharmandayan, Paulo. Campinas. BR
Acta cir. bras ; 36(3): e360304, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1284908
ABSTRACT
ABSTRACT Purpose To evaluate the effect of fractional carbon dioxide (CO2) laser treatment using lower power associated with pulse stacking within collagen fibers, using second harmonic generation microscopy and computerized image analysis. Methods Twenty male Wistar rats aging eight weeks were used. Each treatment area received a single-pass CO2 fractional laser with different parameters. The 20 animals were divided into two groups and euthanized after 30 and 60 days. Second harmonic generation images were obtained and program ImageJ was utilized to evaluate the collagen organization within all areas. Collagen anisotropy, entropy and optical density were quantified. Results Increased anisotropy over time was observed in all four areas, but only reached statistical significance (p = 0.0305) when the mildest parameters were used (area four). Entropy decreased over time in all areas, but without significance(p = 0.1779) in area four. Density showed an overtime increase only in area four, but no statistical significance was reached (p = 0.6534). Conclusions When combined, the results obtained in this study regarding anisotropy, entropy and density tend to demonstrate that it is possible to achieve collagen remodeling with the use of lower power levels associated with stacked pulses.
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Full text: Available Index: LILACS (Americas) Main subject: Skin Aging / Lasers, Gas / Second Harmonic Generation Microscopy Limits: Animals Language: English Journal: Acta cir. bras Year: 2021 Type: Article Institution/Affiliation country: Instituto Federal de Educação, Ciência e Tecnologia de São Paulo/BR / Universidade Estadual de Campinas/BR

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Full text: Available Index: LILACS (Americas) Main subject: Skin Aging / Lasers, Gas / Second Harmonic Generation Microscopy Limits: Animals Language: English Journal: Acta cir. bras Year: 2021 Type: Article Institution/Affiliation country: Instituto Federal de Educação, Ciência e Tecnologia de São Paulo/BR / Universidade Estadual de Campinas/BR