Identification of Proteins Associated with the Formation of Oral Biofilms
Pesqui. bras. odontopediatria clín. integr
;
21: e0128, 2021. tab, graf
Article
in English
| LILACS, BBO
| ID: biblio-1287490
ABSTRACT
ABSTRACT Objective:
To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material andMethods:
Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting.Results:
The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation.Conclusion:
HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Fusobacterium nucleatum
/
Biofilms
/
Genomics
/
Dental Plaque
/
Streptococcus gordonii
Type of study:
Diagnostic study
/
Risk factors
Country/Region as subject:
South America
/
Peru
Language:
English
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Peru
Institution/Affiliation country:
Universidad Nacional Mayor de San Marcos/PE
/
Universidad Peruana Cayetano Heredia/PE
/
Universidad Privada San Juan Bautista/PE
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