Sucrose, Lactose, and Xylitol Exposures Affect Biofilm Formation of Streptococcus mutans
Pesqui. bras. odontopediatria clín. integr
; 21: e0257, 2021. graf
Article
in En
| LILACS, BBO
| ID: biblio-1287491
Responsible library:
BR1264.1
ABSTRACT
Abstract Objective:
To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material andMethods:
This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (a=0.05).Results:
There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p<0.05). An LSD test indicated significant differences among the biofilm formations after exposure to 5% sucrose and 8% lactose and between 5% sucrose and 1% xylitol. In comparison, there were no significant differences (p>0.05) between 8% lactose and 1% xylitol.Conclusion:
Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol.Key words
Full text:
1
Index:
LILACS
Main subject:
Streptococcus mutans
/
Sucrose
/
Xylitol
/
Disaccharides
/
Indonesia
Country/Region as subject:
Asia
Language:
En
Journal:
Pesqui. bras. odontopediatria clín. integr
Journal subject:
ODONTOLOGIA
Year:
2021
Type:
Article