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Immunological Characteristics between αß TDC and γδ TDC Cells in the Spleen of Breast Cancer-Induced Mice / Características imunológicas entre células TDC αß e TDC γδ no baço de camundongos com câncer de mama induzido
Silva, Polyana Barbosa; Michelin, Márcia Antoniazi; Jammal, Millena Prata; Murta, Eddie Fernando Cândido.
  • Silva, Polyana Barbosa; Universidade Federal do Triângulo Mineiro. Reseach Institute of Oncology. Uberaba. BR
  • Michelin, Márcia Antoniazi; Universidade Federal do Triângulo Mineiro. Reseach Institute of Oncology. Uberaba. BR
  • Jammal, Millena Prata; Universidade Federal do Triângulo Mineiro. Reseach Institute of Oncology. Uberaba. BR
  • Murta, Eddie Fernando Cândido; Universidade Federal do Triângulo Mineiro. Reseach Institute of Oncology. Uberaba. BR
Rev. bras. ginecol. obstet ; 43(5): 368-373, May 2021. graf
Article in English | LILACS | ID: biblio-1288556
ABSTRACT
Abstract Objective To evaluate the antitumoral role of γδ TDC cells and αβ TDC cells in an experimental model of breast cancer. Methods Thirty female Balb/c mice were divided into 2 groups control group (n=15) and induced-4T1 group (n=15), in which the mice received 2 x 105 4T1 mammary tumor cell line. Following the 28-day experimental period, immune cells were collected from the spleen and analyzed by flow cytometry for comparison of αβ TDC (TCRαβ+ CD11c+MHCII+) and γδ TDC (TCRγδ+CD11c+MHCII+) cells regarding surface markers (CD4+ and C8+) and cytokines (IFN-γ, TNF-α, IL-12 and IL-17). Results A total of 26.53% of γδ TDC- control group (p<0.0001) - the proportion of αβ TDC was lower in splenic cells than γδ TDC; however, these 2 cell types were reduced in tumor conditions (p<0.0001), and the proportion of IFN-γ, TNF-α, IL-12 and IL-17 cytokines produced by γδ TDC was higher than those produced by αβ TDC, but it decreased under conditions of tumor-related immune system response (p<0.0001). Conclusion Healthy mice engrafted with malignant cells 4T1 breast tumor presented TDC with γδ TCR repertoire. These cells express cytotoxic molecules of lymphocytes T, producing anti-tumor proinflammatory cytokines.
RESUMO
Resumo Objetivo Esclarecer o possível papel antitumoral das células TDC γδ e TDC αβ em um modelo experimental de câncer de mama. Métodos Trinta baços de camundongos Balb/c analisados por citometria de fluxo, separados entre grupo controle (n=15) e o grupo tumoral induzido por 4T1 (n=15). Resultados Presença de 26,53% de TDC γδ nos camundongos do grupo controle (p<0,0001), proporção de TDC αβ menor em células esplênicas do que TDC γδ; no entanto, estes dois tipos de células são reduzidos emcondições tumorais (p<0,0001), e a proporção de citocinas IFN-γ, TNF-α, IL-12 e IL-17 produzidas pelas célula TDC γδ foi maior do que as produzidas pelas células TDC αβ, mas foram diminuídas sob condições de resposta ao sistema imunológico relacionada ao tumor (p<0,0001). Conclusão Camundongos saudáveis induzidos ao tumor de mama 4T1 apresentaram TDC com repertório TCR γδ. Estas células expressam moléculas citotóxicas de linfócitos T, produzindo citocinas proinflamatórias anti-tumor.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / Receptors, Antigen, T-Cell, gamma-delta / Receptors, Antigen, T-Cell, alpha-beta Type of study: Prognostic study Limits: Animals Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Triângulo Mineiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / Receptors, Antigen, T-Cell, gamma-delta / Receptors, Antigen, T-Cell, alpha-beta Type of study: Prognostic study Limits: Animals Language: English Journal: Rev. bras. ginecol. obstet Journal subject: Gynecology / Obstetrics Year: 2021 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Triângulo Mineiro/BR