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Transcriptional analysis of Rhazya stricta in response to jasmonic acid
Hajrah, Nahid H; Rabah, Samar O; Alghamdi, Mohammed K; Atef, Ahmed; Edris, Sherif; Alhebshi, Alawiah M; Hassan, Sabah M; Alzahrani, Dhafer A; Bahieldin, Ahmed; Mutwakil, Mohammed HZ; Alqurashi, Yaser E; Al-Zahrani, Hassan S; Abo-Aba, Salah EM; Jansen, Robert K; Sabir, Jamal SM; Hall, Neil; Rasool Kamli, Majid.
  • Hajrah, Nahid H; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Rabah, Samar O; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Alghamdi, Mohammed K; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Atef, Ahmed; King Abdulaziz University. Center of Excellence in Bionanoscience Research. Jeddah. SA
  • Edris, Sherif; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Alhebshi, Alawiah M; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Hassan, Sabah M; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Alzahrani, Dhafer A; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Bahieldin, Ahmed; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Mutwakil, Mohammed HZ; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Alqurashi, Yaser E; Majmaah University. College of Science. Department of Biology. Majmaah. SA
  • Al-Zahrani, Hassan S; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Abo-Aba, Salah EM; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Jansen, Robert K; University of Texas at Austin. Department of Integrative Biology. US
  • Sabir, Jamal SM; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Hall, Neil; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Rasool Kamli, Majid; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
Electron. j. biotechnol ; 50: 68-76, Mar. 2021. ilus, tab, graf
Article in English | LILACS | ID: biblio-1292417
ABSTRACT

BACKGROUND:

Jasmonic acid (JA) is a signal transducer molecule that plays an important role in plant development and stress response; it can also efficiently stimulate secondary metabolism in plant cells.

RESULTS:

RNA-Seq technology was applied to identify differentially expressed genes and study the time course of gene expression in Rhazya stricta in response to JA. Of more than 288 million total reads, approximately 27% were mapped to genes in the reference genome. Genes involved during the secondary metabolite pathways were up- or downregulated when treated with JA in R. stricta. Functional annotation and pathway analysis of all up- and downregulated genes identified many biological processes and molecular functions. Jasmonic acid biosynthetic, cell wall organization, and chlorophyll metabolic processes were upregulated at days 2, 6, and 12, respectively. Similarly, the molecular functions of calcium-transporting ATPase activity, ADP binding, and protein kinase activity were also upregulated at days 2, 6, and 12, respectively. Time-dependent transcriptional gene expression analysis showed that JA can induce signaling in the phenylpropanoid and aromatic acid pathways. These pathways are responsible for the production of secondary metabolites, which are essential for the development and environmental defense mechanism of R. stricta during stress conditions.

CONCLUSIONS:

Our results suggested that genes involved in flavonoid biosynthesis and aromatic acid synthesis pathways were upregulated during JA stress. However, monoterpenoid indole alkaloid (MIA) was unaffected by JA treatment. Hence, we can postulate that JA plays an important role in R. stricta during plant development and environmental stress conditions.
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Full text: Available Index: LILACS (Americas) Main subject: Cyclopentanes / Apocynaceae / Oxylipins Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: Saudi Arabia / United States Institution/Affiliation country: King Abdulaziz University/SA / Majmaah University/SA / University of Texas at Austin/US

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Full text: Available Index: LILACS (Americas) Main subject: Cyclopentanes / Apocynaceae / Oxylipins Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: Saudi Arabia / United States Institution/Affiliation country: King Abdulaziz University/SA / Majmaah University/SA / University of Texas at Austin/US