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Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro
Jiang, Ruijiao; Zhang, Pengfei; Wu, Xulong; Wang, Yin; Rehman, Tayyab; Yao, Xueping; Luo, Yan; Yang, Zexiao.
  • Jiang, Ruijiao; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
  • Zhang, Pengfei; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
  • Wu, Xulong; Chengdu Agricultural College. Branch of Animal Husbandry and Veterinary Medicine. Chengdu. CN
  • Wang, Yin; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
  • Rehman, Tayyab; Sichuan Agricultural University. College of Basic Veterinary Medicine. Chengdu. CN
  • Yao, Xueping; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
  • Luo, Yan; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
  • Yang, Zexiao; Sichuan Agricultural University. College of Veterinary Medicine. Sichuan Provincial Key Laboratory of Animal Diseases and Human Health. Chengdu. CN
Electron. j. biotechnol ; 50: 16-22, Mar. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1292419
Responsible library: CL1.1
ABSTRACT

BACKGROUND:

Cecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.

RESULTS:

The results indicated that the recombinant protein was about 5.5 kDa showed by Tricine­SDS­ PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.

CONCLUSIONS:

Collectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.
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Full text: Available Index: LILACS (Americas) Main subject: Peptides / Saccharomyces cerevisiae / Anti-Bacterial Agents Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Chengdu Agricultural College/CN / Sichuan Agricultural University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Peptides / Saccharomyces cerevisiae / Anti-Bacterial Agents Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Chengdu Agricultural College/CN / Sichuan Agricultural University/CN