Atractylenolide III alleviates sepsis-mediated lung injury via inhibition of FoxO1 and VNN1 protein
Acta cir. bras
;
36(8): e360802, 2021. graf
Article
in English
| LILACS, VETINDEX
| ID: biblio-1339011
ABSTRACT
ABSTRACT Purpose:
To evaluate the influence of atractylenolide (Atr) III on sepsis-induced lung damage.Methods:
We constructed a mouse sepsis model through cecal ligation and puncture. These mice were allocated to the normal, sepsis, sepsis + Atr III-L (2 mg/kg), as well as Atr III-H (8 mg/kg) group. Lung injury and pulmonary fibrosis were accessed via hematoxylin-eosin (HE) and Masson's staining. We used terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and flow cytometry for detecting sepsis-induced lung cell apoptosis. The contents of the inflammatory cytokines in lung tissue were measured via enzyme-linked immunosorbent assay (ELISA).Results:
Atr III-H did not only reduce sepsis-induced lung injury and apoptosis level, but also curbed the secretion of inflammatory factors. Atr III-H substantially ameliorated lung function and raised Bcl-2 expression. Atr III-H eased the pulmonary fibrosis damage and Bax, caspase-3, Vanin-1 (VNN1), as well as Forkhead Box Protein O1 (FoxO1) expression.Conclusions:
Atr III alleviates sepsis-mediated lung injury via inhibition of FoxO1 and VNN1 protein.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sesquiterpenes
/
Sepsis
/
Lung Injury
/
Forkhead Box Protein O1
/
Amidohydrolases
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2021
Type:
Article
Institution/Affiliation country:
Institute of Guangzhou Medical University/CN
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