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Optimal culture conditions for neurosphere formation and neuronal differentiation from human dental pulp stem cells
Gonmanee, Thanasup; Arayapisit, Tawepong; Vongsavan, Kutkao; Phruksaniyom, Chareerut; Sritanaudomchai, Hathaitip.
  • Gonmanee, Thanasup; Mahidol University. Faculty of Medicine Ramathibodi Hospital. Chakri Naruebodindra Medical Institute. Samut Prakan. TH
  • Arayapisit, Tawepong; Mahidol University. Faculty of Dentistry. Department of Anatomy. Bangkok. TH
  • Vongsavan, Kutkao; Walailak University. International College of Dentistry. Department of Pediatric Dentistry. Bangkok. TH
  • Phruksaniyom, Chareerut; Mahidol University. Faculty of Dentistry. Department of Pharmacology. Bangkok. TH
  • Sritanaudomchai, Hathaitip; Mahidol University. Faculty of Dentistry. Department of Oral Biology. Bangkok. TH
J. appl. oral sci ; 29: e20210296, 2021. graf
Article in English | LILACS | ID: biblio-1340101
Responsible library: BR1.1
ABSTRACT
Abstract Objectives Human dental pulp stem cells (DPSCs) have been used to regenerate damaged nervous tissues. However, the methods of committing DPSCs into neural stem/progenitor cells (NSPCs) or neurospheres are highly diverse, resulting in many neuronal differentiation outcomes. This study aims to validate an optimal protocol for inducing DPSCs into neurospheres and neurons. Methodology After isolation and characterization of mesenchymal stem cell identity, DPSCs were cultured in a NSPC induction medium and culture vessels. The durations of the culture, dissociation methods, and passage numbers of DPSCs were varied. Results Neurosphere formation requires a special surface that inhibits cell attachment. Five-days was the most appropriate duration for generating proliferative neurospheres and they strongly expressed Nestin, an NSPC marker. Neurosphere reformation after being dissociated by the Accutase enzyme was significantly higher than other methods. Passage number of DPSCs did not affect neurosphere formation, but did influence neuronal differentiation. We found that the cells expressing a neuronal marker, β-tubulin III, and exhibiting neuronal morphology were significantly higher in the early passage of the DPSCs. Conclusion These results suggest a guideline to obtain a high efficiency of neurospheres and neuronal differentiation from DPSCs for further study and neurodegeneration therapeutics.
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Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Dental Pulp Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Thailand Institution/Affiliation country: Mahidol University/TH / Walailak University/TH

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Full text: Available Index: LILACS (Americas) Main subject: Stem Cells / Dental Pulp Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2021 Type: Article Affiliation country: Thailand Institution/Affiliation country: Mahidol University/TH / Walailak University/TH