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Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger
Idrissa, H; Abdoulaye, O; Yacouba, A; Alhousseini Maiga, D; Moumouni Sambo, H; Doutchi, M; Chaibou, S; Sani, O; Mamadou, S; Sangare, L.
  • Idrissa, H; Direction des Laboratoires de santé (DLS), Ministère de la santé publique. Niamey. NE
  • Abdoulaye, O; Faculté des Sciences de la Santé, Université Dan Dicko Dankoulodo. Maradi. NE
  • Yacouba, A; Faculté des Sciences de la Santé, Université Abdou Moumouni. Niamey. NE
  • Alhousseini Maiga, D; Faculté des Sciences de la Santé, Université Abdou Moumouni. Niamey. NE
  • Moumouni Sambo, H; Direction des Laboratoires de santé (DLS), Ministère de la santé publique. Niamey. NE
  • Doutchi, M; Faculté des Sciences de la Santé, Université de Zinder. Niamey. NE
  • Chaibou, S; Hôpital Général de Référence de Niamey. Niamey. NE
  • Sani, O; Centre de Recherche Médicale et Sanitaire. Niamey. NE
  • Mamadou, S; Faculté des Sciences de la Santé, Université Abdou Moumouni. Niamey. NE
  • Sangare, L; Unité de Formation et de Recherhe en Sciences de la Santé (UFR/SDS), Université Joseph Ki-Zerbo. Ouagadougou. BF
Afr. J. Clin. Exp. Microbiol ; 22(4): 448-456, 2021.
Article in English | AIM | ID: biblio-1342108
Responsible library: CG1.1
ABSTRACT

Background:

Risk assessment is the means of identifying and evaluating potential errors or problems that may occur in testing process. The aim of this study was to perform risk assessment of antimicrobial susceptibility testing (AST) process in clinical microbiology laboratories of Niamey, Niger Republic.

Methodology:

We conducted a descriptive cross-sectional study from October 1 to December 31, 2019, to evaluate AST performance in seven clinical microbiology laboratories at Niamey, the capital city of Niger republic. The evaluation focused on the determination of the criticality index (CI) of each critical point (frequency of occurrence of anomalies, severity of the process anomaly, and detectability of the anomaly during the process) in the AST process and the performance of the AST through an observation sheet using two reference strains; Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.

Results:

The criticality index (CI) was greater than 6 for most of the critical points related to material, medium, equipment, method and labour for the AST process in all the laboratories. A range of 18-100% errors on the inhibition zone diameters of the reference strains were observed. Major and/or minor categorization (Sensitive S, Intermediate I and Resistance R) discrepancies were found at all the laboratories for either one or both reference strains. The antibiotics most affected by the S/I/R discrepancies were trimethoprim (100%), vancomycin (100%), amoxicillin (80%) and amoxicillin + clavulanic acid (70%).

Conclusion:

This study showed a deficiency in the control of critical control points that impacts the performance of the AST reported by the laboratories in Niger. Corrective actions are needed to improve the performance of AST in clinical microbiology laboratories in Niger
Subject(s)


Full text: Available Index: AIM (Africa) Main subject: Quality Control / Microbial Sensitivity Tests / Medical Laboratory Science / Microbiology Type of study: Risk factors Limits: Humans Country/Region as subject: Africa Language: English Journal: Afr. J. Clin. Exp. Microbiol Year: 2021 Type: Article Institution/Affiliation country: Centre de Recherche Médicale et Sanitaire/NE / Direction des Laboratoires de santé (DLS), Ministère de la santé publique/NE / Faculté des Sciences de la Santé, Université Abdou Moumouni/NE / Faculté des Sciences de la Santé, Université Dan Dicko Dankoulodo/NE / Faculté des Sciences de la Santé, Université de Zinder/NE / Hôpital Général de Référence de Niamey/NE / SDS), Université Joseph Ki-Zerbo+BF

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Full text: Available Index: AIM (Africa) Main subject: Quality Control / Microbial Sensitivity Tests / Medical Laboratory Science / Microbiology Type of study: Risk factors Limits: Humans Country/Region as subject: Africa Language: English Journal: Afr. J. Clin. Exp. Microbiol Year: 2021 Type: Article Institution/Affiliation country: Centre de Recherche Médicale et Sanitaire/NE / Direction des Laboratoires de santé (DLS), Ministère de la santé publique/NE / Faculté des Sciences de la Santé, Université Abdou Moumouni/NE / Faculté des Sciences de la Santé, Université Dan Dicko Dankoulodo/NE / Faculté des Sciences de la Santé, Université de Zinder/NE / Hôpital Général de Référence de Niamey/NE / SDS), Université Joseph Ki-Zerbo+BF