O perfil epidemiológico e o desempenho da GAL-3 e CK-19 em lesões foliculares e suspeitas para malignidade da tireoide / The epidemiological profile and the performance of GAL-3 and CK-19 in follicular lesions and suspicious for malignancy of the thyroid

RESUMO

Objetivos: Analisar dados epidemiológicos de pacientes submetidos à PAAF, classificados como lesões foliculares (classe IV) e suspeitos para malignidade (classe V) pelo Sistema Bethesda para Laudos Citopatológicos da Tireoide e avaliar o desempenho do painel imunocitoquímico com os marcadores CK-19, Galectina-3 e HBME-1 no diagnóstico destes pacientes. Metodologia: Estudo retrospectivo transversal, que analisou 24 laudos anatomopatológicos do Centro de Patologia de Curitiba, entre 2011 e 2014, classificados como classe IV e V do Sistema Bethesda, cujas lâminas foram avaliadas pela imunocitoquímica, usando-se os marcadores CK-19, GAL-3 e HBME-1. Resultados: 83,3% dos pacientes eram mulheres, com idade média de 45,7 anos. O tamanho médio das lesões foi 12,5mm, localizadas 56,5% no lobo D. O painel de marcadores apresentou sensibilidade 100%, especificidade 90,5% e eficácia 95,5% para as lesões avaliadas. Conclusão: A imunocitoquímica demostrou-se um exame pré-operatório eficaz para diferenciação de lesões foliculares e suspeitas para malignidade do carcinoma papilífero da tireoide

ABSTRACT

Objectives: To analyze epidemiological data of patients who undergone FNA that was classified as follicular lesions (Class IV) and suspicious for malignancy (Class V) by the Bethesda System for Reporting Thyroid Cytopathology and evaluate the performance of immunocytochemical panel with markers CK-19, Galectin-3 and HBME-1 in these patients. Methodology: This is a cross-sectional retrospective study. It was analyzed 24 pathological between 2011 and 2014, from the Curitiba Pathology Center, classified as Class IV and V in the Bethesda System for Reporting Thyroid Cytopathology, which slides were stained with immunocytochemistry using the markers CK19 GAL-13 and HBME-1. Results: In this study 83.3% of the patients were women with a mean age of 45.7 years. The average size of the nodules was 12.5mm and 56.5% was located in the right lobe. Regarding the diagnosis of follicular lesions and suspicious for malignancy, the sensitivity of the exam with the standard biological markers used was 100%, specificity 90,5% and efficacy 95.5%. Conclusion: The immunocytochemistry demonstrated to be an effective pre-operative exam for differentiation of follicular lesions and suspicious for malignancy of papillary thyroid carcinoma