Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold
Acta cir. bras
;
36(10): e361001, 2021. graf
Article
in English
| LILACS, VETINDEX
| ID: biblio-1345021
ABSTRACT
ABSTRACT Purpose:
To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits.Methods:
The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decellularization process using 1% sodium dodecyl sulfate (SDS) or 2% sodium deoxycholate (SD) to compose 12 study groups (G) pure SD or SDS, exposed or not to 1% TritonX-100 (TX-100) and exposed or not to poly-l'lysine and laminin (PL). Scaffolds were covered with 1 × 105 or 1 × 106 ASCs diluted in 10 μL Puramatrix™. The histological analysis was done by cell counting in hematoxylin and eosin (HE) and nuclei count in immunofluorescence (IF) with 4',6-Diamidine-2'-phenylindole dihydrochloride (DAPI).Results:
The study of groups in HE and IF showed similar results. For both analyses,IVC-SD-1 × 106 ASC and IVC-SD-PL-1 × 106 ASC provided the best results. The IF technique showed better sensitivity than HE, with a weak agreement between them.Conclusions:
Decellularizing agent and the number of ASC influence scaffolds cellularization response and the best protocols as those ones using SD with or without the addition of PL.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mesenchymal Stem Cells
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2021
Type:
Article
Institution/Affiliation country:
Universidade Estadual Paulista/BR
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