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Toxicity of Carnoy's solution toward human keratinocytes: an in vitro study
Pinto Júnior, Aécio Abner Campos; Viana, Karolina Skarlet Silva; Abreu, Lucas Guimarães; Nascentes, Clésia Cristina; Diniz, Ivana Márcia Alves; Mesquita, Ricardo Alves; Bernardes, Vanessa Fátima.
Affiliation
  • Pinto Júnior, Aécio Abner Campos; Centro Universitário de Belo Horizonte. College of Dentistry. Belo Horizonte. BR
  • Viana, Karolina Skarlet Silva; Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte. BR
  • Abreu, Lucas Guimarães; Universidade Federal de Minas Gerais. School of Dentistry. Department of Childs and Adolescents Oral Health. Belo Horizonte. BR
  • Nascentes, Clésia Cristina; Universidade Federal de Minas Gerais. Institute of Mathematical Sciences. Department of Chemistry. Belo Horizonte. BR
  • Diniz, Ivana Márcia Alves; Universidade Federal de Minas Gerais. School of Dentistry. Department of Restorative Dentistry. Belo Horizonte. BR
  • Mesquita, Ricardo Alves; Universidade Federal de Minas Gerais. School of Dentistry. Department of Oral Surgery and Pathology. Belo Horizonte. BR
  • Bernardes, Vanessa Fátima; Universidade Federal de Minas Gerais. Biological Sciences Institute. Department of Pathology. Belo Horizonte. BR
Braz. oral res. (Online) ; 35: e124, 2021. tab, graf
Article in En | LILACS-Express | LILACS, BBO | ID: biblio-1350358
Responsible library: BR1.1
ABSTRACT
Abstract The present study aimed to characterize the chemical elements and cytotoxicity of Carnoy's solution (CS) by comparing two different trademarked products (one Brazilian [NCS] and another imported [ICS]) using inductively coupled plasma mass spectrometry (ICP-MS) and human keratinocyte (HaCaT) cultures. For performing ICP-MS, the solutions were diluted according to calibration curves, and the chemical elements were analyzed with a spectrometer. HaCaT cells were exposed to CS concentrations ranging from 0.10% to 20% for 3 or 5 min. Cell viability was evaluated immediately (T0), 24 h (T1), and 7 days (T2) after exposure to CS using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) reduction assay. Data were analyzed using a t-test for ICP-MS and analysis of variance followed by Tukey's post-hoc test for MTT assay, both considering statistical significance at p<0.05. ICP-MS results revealed that ICS presented significantly lower concentrations of 12 chemical elements than NCS. The results of MTT assay revealed that at T0, ICS was more cytotoxic than NCS regardless of the time of exposure (p < 0.05). At T1, the only difference between the groups was at a concentration of 0.10% after 5 min of exposure. At T2, at a concentration of 0.5%, ICS resulted in a significant reduction in cell viability compared to NCS (p < 0.05). Thus, the results showed that ICS was more cytotoxic than NCS. Collectively, our findings suggest that the individual compositions of different CS formulations should be investigated.
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Full text: 1 Index: LILACS Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2021 Type: Article / Project document

Full text: 1 Index: LILACS Language: En Journal: Braz. oral res. (Online) Journal subject: ODONTOLOGIA Year: 2021 Type: Article / Project document