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Recovery of motor function in rats with complete spinal cord injury following implantation of collagen/silk fibroin scaffold combined with human umbilical cord-mesenchymal stem cells
Deng, Wu-Sheng; Liu, Xiao-Yin; Ma, Ke; Liang, Bing; Liu, Ying-Fu; Wang, Ren-Jie; Chen, Xu-Yi; Zhang, Sai.
  • Deng, Wu-Sheng; University of Chinese Medicine. Gansu Province. CN
  • Liu, Xiao-Yin; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
  • Ma, Ke; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
  • Liang, Bing; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
  • Liu, Ying-Fu; Cangzhou nanobody technology innovation center. Cangzhou. CN
  • Wang, Ren-Jie; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
  • Chen, Xu-Yi; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
  • Zhang, Sai; Hospital Brain Center. Tianjin Key Laboratory of Neurotrauma Repair. Tianjin. CN
Rev. Assoc. Med. Bras. (1992) ; 67(9): 1342-1348, Sept. 2021. graf
Article in English | LILACS | ID: biblio-1351459
ABSTRACT
SUMMARY

OBJECTIVE:

This study aimed to assess the effect of the collagen/silk fibroin scaffolds seeded with human umbilical cord-mesenchymal stem cells on functional recovery after acute complete spinal cord injury.

METHODS:

The fibroin and collagen were mixed (mass ratio, 37), and the composite scaffolds were produced. Forty rats were randomly divided into the Sham group (without spinal cord injury), spinal cord injury group (spinal cord transection without any implantation), collagen/silk fibroin scaffolds group (spinal cord transection with implantation of the collagen/silk fibroin scaffolds), and collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (spinal cord transection with the implantation of the collagen/silk fibroin scaffolds co-cultured with human umbilical cord-mesenchymal stem cells). Motor evoked potential, Basso-Beattie-Bresnahan scale, modified Bielschowsky's silver staining, and immunofluorescence staining were performed.

RESULTS:

The BBB scores in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group were significantly higher than those in the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). The amplitude and latency were markedly improved in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). Meanwhile, compared to the spinal cord injury and collagen/silk fibroin scaffolds groups, more neurofilament positive nerve fiber ensheathed by myelin basic protein positive structure at the injury site were observed in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (p<0.01, p<0.05). The results of Bielschowsky's silver staining indicated more nerve fibers was observed at the lesion site in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.01, p< 0.05).

CONCLUSION:

The results demonstrated that the transplantation of human umbilical cord-mesenchymal stem cells on a collagen/silk fibroin scaffolds could promote nerve regeneration, and recovery of neurological function after acute spinal cord injury.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Spinal Cord Injuries / Mesenchymal Stem Cell Transplantation / Mesenchymal Stem Cells / Fibroins Limits: Animals / Humans Language: English Journal: Rev. Assoc. Med. Bras. (1992) Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Cangzhou nanobody technology innovation center/CN / Hospital Brain Center/CN / University of Chinese Medicine/CN

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Full text: Available Index: LILACS (Americas) Main subject: Spinal Cord Injuries / Mesenchymal Stem Cell Transplantation / Mesenchymal Stem Cells / Fibroins Limits: Animals / Humans Language: English Journal: Rev. Assoc. Med. Bras. (1992) Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Cangzhou nanobody technology innovation center/CN / Hospital Brain Center/CN / University of Chinese Medicine/CN