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Ameliorative effects of Lactobacillus against Aflatoxin B1 / Efeitos benéficos do Lactobacillus contra a Aflatoxin B1
Yasmeen, R; Zahid, B; Alyas, S; Akhtar, R; Zahra, N; Kouser, S; Hashmi, A S; Athar, M; Tayyab, M; Anjum, A A.
  • Yasmeen, R; KBCMA College of Veterinary and Animal Sciences. Narowal. PK
  • Zahid, B; KBCMA College of Veterinary and Animal Sciences. Narowal. PK
  • Alyas, S; University of Lahore. Institute of Molecular Biology and Biotechnology. Punjab. PK
  • Akhtar, R; University of Veterinary and Animal Sciences. Departments of Pathology. Lahore. PK
  • Zahra, N; University of Lahore. Institute of Molecular Biology and Biotechnology. Punjab. PK
  • Kouser, S; University of Veterinary and Animal Sciences. Departments of Microbiology. Lahore. PK
  • Hashmi, A S; Riphah College of Veterinary Sciences. Lahore. PK
  • Athar, M; Hi-Tech Feeds. Lahore. PK
  • Tayyab, M; University of Veterinary and Animal Sciences. Institute of Biochemistry and Biotechnology. Lahore. PK
  • Anjum, A A; University of Veterinary and Animal Sciences. Departments of Microbiology. Lahore. PK
Braz. j. biol ; 84: e250517, 2024. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1355859
ABSTRACT
Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactobacillus from broiler gut were incubated with 100 ppb AFB1 in aqueous environment and effect of different parameters (cell fractions, time, temperature, pH) on detoxification was determined by HPLC. The ameliorative effect of Lactobacillus salivarius (LS) against AFB1 was studied in broiler. The results revealed that LS (CR. 4) showed the best results (in vitro) as compared to other isolates (L. salivarius (CR. 3, CR, 4), L. agilis (CE. 2.1, CE. 3.1) and L. crispatus (CE. 28). Cell debris of CR. 4 showed significantly higher detoxification (P<0.05). Maximum amount of AFB1 was detoxified at 30°C (97%), pH 4.0 (99%) and 6 h (99.97%). In vivo study showed that AFB1 decreased weight gain (1,269 ± 0.04 gm/ bird), feed consumed (2,161 ± 0.08 gm/ bird), serum total protein (2.42 ± 0.34 gm/ dl), serum albumin (0.5 ± 0.2 2 gm/dl) and antibody titer (4.2 ± 0.83). Liver function enzymes were found (alanine transaminase (ALT) 32 ± 10.7 U/L) and aspartate transaminase (AST) 314.8 ± 27 U/L) elevated in AFB1 fed broilers. Treatment with 1% LS not only decreased the toxic effects of AFB1 (group D) but also improved the overall health of broilers due to its probiotic effects (p<0.05) as compared to control negative (group A). The detoxification ability of LS was better than commercial binder (CB) (0.2% Protmyc). It was concluded that detoxification of AFB1 by Lactobacillus was strain, temperature, pH and time dependent. LS has detoxification ability against AFB1 in vivo.
RESUMO
Resumo Os lactobacilos são probióticos com capacidade de desintoxicação da Aflatoxina (AF), encontrados em produtos fermentados, TGI de animais e meio ambiente. O objetivo deste estudo foi investigar a capacidade de isolados de frango de corte de Lactobacillus contra a Aflatoxina B1 (AFB1). Para tanto, 5 isolados de Lactobacillus de intestino de frango foram incubados com 100 ppb AFB1 em meio aquoso, e o efeito de diferentes parâmetros (frações celulares, tempo, temperatura, pH) na desintoxicação foi determinado por CLAE. O efeito melhorador de Lactobacillus salivarius (LS) contra AFB1 foi estudado em frangos de corte. Os resultados revelaram que LS (CR. 4) apresentou os melhores resultados (in vitro) em comparação com outros isolados [L. salivarius (CR. 3, CR. 4), L. agilis (CE. 2.1, CE. 3.1) e L. crispatus (CE. 28)]. Detritos celulares de CR. 4 mostraram desintoxicação significativamente maior (P < 0.05). A quantidade máxima de AFB1 foi desintoxicada a 30 °C (97%), pH 4.0 (99%) e 6 h (99,97%). O estudo in vivo mostrou que AFB1 diminuiu o ganho de peso (1,269 ± 0.04 g / ave), alimento consumido (2,161 ± 0.08 g / ave), proteína total sérica (2.42 ± 0.34 g / dl), albumina sérica (0.5 ± 0.22 gm / dl) e título de anticorpo (4.2 ± 0.83). As enzimas da função hepática foram encontradas (alanina transaminase (ALT) 32 ± 10.7 U / L) e aspartato transaminase (AST) 314.8 ± 27 U / L) elevadas em AFB1 alimentados com frangos. O tratamento com 1% LS não só diminuiu os efeitos tóxicos de AFB1 (grupo D), mas também melhorou a saúde geral dos frangos devido aos seus efeitos probióticos (p < 0.05) em comparação com o controle negativo (grupo A). A capacidade de desintoxicação do LS foi melhor do que o aglutinante comercial (CB) (0.2% Protmyc). Concluiu-se que a desintoxicação de AFB1 por Lactobacillus foi dependente da cepa, temperatura, pH e tempo. LS tem capacidade de desintoxicação contra AFB1 in vivo.

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Full text: Available Index: LILACS (Americas) Main subject: Aflatoxin B1 / Probiotics Limits: Animals Language: English Journal: Braz. j. biol Year: 2024 Type: Article Institution/Affiliation country: Hi-Tech Feeds/PK / KBCMA College of Veterinary and Animal Sciences/PK / Riphah College of Veterinary Sciences/PK / University of Lahore/PK / University of Veterinary and Animal Sciences/PK

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Full text: Available Index: LILACS (Americas) Main subject: Aflatoxin B1 / Probiotics Limits: Animals Language: English Journal: Braz. j. biol Year: 2024 Type: Article Institution/Affiliation country: Hi-Tech Feeds/PK / KBCMA College of Veterinary and Animal Sciences/PK / Riphah College of Veterinary Sciences/PK / University of Lahore/PK / University of Veterinary and Animal Sciences/PK